PMID- 8897700
OWN - NLM
STAT- MEDLINE
DCOM- 19970304
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 84
IP  - 2
DP  - 1996 Oct 15
TI  - DHG, a new depolymerized holothurian glycosaminoglycan, exerts an antithrombotic 
      effect with less bleeding than unfractionated or low molecular weight heparin, in 
      rats.
PG  - 111-20
AB  - We characterized the antithrombotic, haemorrhagic, and ex vivo anticoagulant 
      effects of a recently identified depolymerized holothurian glycosaminoglycan 
      (DHG), and compared these effects with those of unfractionated heparin (UFH), low 
      molecular weight heparin (LMWH), and dermatan sulfate (DS). In thrombin-induced 
      venous thrombus formation in rats, DHG had a significant preventive effect at 0.3 
      mg/kg or more at 5 min after i.v., administration. UFH, LMWH, and DS also showed 
      a significant antithrombotic effect at 0.3, 0.3, and 1 mg/kg, respectively, under 
      the same experimental conditions. After rat tail transection, DHG, UFH, LMWH, and 
      DS prolonged the bleeding time significantly at 10, 1, 1, and 10 mg/kg, 
      respectively, at 5 min after i.v., injection. Therefore, DHG exerts its 
      antithrombotic effect with less bleeding than UFH and LMWH in experimental 
      animals. DHG prolonged the activated partial thromboplastin time in a 
      dose-dependent manner at 0.3-3 mg/kg, at which dose an antithrombotic effect was 
      exhibited without any significant haemorrhagic effect. All of these 
      glycosaminoglycans prolonged thrombin clotting time markedly at their 
      haemorrhagic doses.
FAU - Kitazato, K
AU  - Kitazato K
AD  - Pharmacology Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, 
      Japan.
FAU - Kitazato, K T
AU  - Kitazato KT
FAU - Nagase, H
AU  - Nagase H
FAU - Minamiguchi, K
AU  - Minamiguchi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Anticoagulants)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Heparin, Low-Molecular-Weight)
SB  - IM
MH  - Animals
MH  - Anticoagulants/adverse effects/chemistry/*pharmacology
MH  - Blood Coagulation/*drug effects
MH  - Glycosaminoglycans/adverse effects/chemistry/*pharmacology
MH  - Hemorrhage/chemically induced
MH  - Heparin, Low-Molecular-Weight/pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
EDAT- 1996/10/15 00:00
MHDA- 1996/10/15 00:01
CRDT- 1996/10/15 00:00
PHST- 1996/10/15 00:00 [pubmed]
PHST- 1996/10/15 00:01 [medline]
PHST- 1996/10/15 00:00 [entrez]
AID - 0049-3848(96)00166-1 [pii]
AID - 10.1016/0049-3848(96)00166-1 [doi]
PST - ppublish
SO  - Thromb Res. 1996 Oct 15;84(2):111-20. doi: 10.1016/0049-3848(96)00166-1.