PMID- 8897990
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20180109
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 271
IP  - 4 Pt 2
DP  - 1996 Oct
TI  - Adenosine causes cAMP-dependent activation of chick embryo red cell carbonic 
      anhydrase and 2,3-DPG synthesis.
PG  - R973-81
AB  - In late chick embryos, coordinate activation of red cell carbonic anhydrase II 
      (CAII) and 2,3-diphosphoglycerate (2,3-DPG) synthesis is initiated by hypoxia. 
      The effects are mediated by unidentified hormonal effectors resident in chick 
      plasma. In the present investigation, we have analyzed the effect of adenosine 
      receptor stimulation on embryonic red cell CAII and 2,3-DPG synthesis. We find 
      that primitive and definitive embryonic red blood cells from chick have an A2a 
      adenosine receptor. Stimulation of the receptor with metabolically stable 
      adenosine analogues causes a large increase of red cell adenosine 3',5'-cyclic 
      monophosphate (cAMP) and subsequent activation of red cell CAII and 2,3-DPG 
      production in definitive red blood cells and of CAII synthesis in primitive red 
      blood cells. Direct stimulation of adenylyl cyclase with forskolin has the same 
      effect. Analysis of red cell protein pattern after labeling with [35S]methionine 
      shows that stimulation of red cell cAMP levels activates synthesis of several 
      other proteins aside from CAII. Presence of actinomycin D inhibits cAMP-dependent 
      changes of protein synthesis, indicating that cAMP-dependent transcriptional 
      activation is required. In contrast to the stable adenosine receptor analogues, 
      adenosine itself was a very weak agonist, unless its metabolism was significantly 
      inhibited. Thus, besides adenosine, other effectors of the adenylyl cyclase 
      system are likely to be involved in the O2 pressure-dependent regulation of red 
      cell metabolism in late development of avian embryos.
FAU - Glombitza, S
AU  - Glombitza S
AD  - Physiologisches Institut, Universitat Regensburg, Germany.
FAU - Dragon, S
AU  - Dragon S
FAU - Berghammer, M
AU  - Berghammer M
FAU - Pannermayr, M
AU  - Pannermayr M
FAU - Baumann, R
AU  - Baumann R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Adenosine Deaminase Inhibitors)
RN  - 0 (Diphosphoglyceric Acids)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nucleosides)
RN  - 0 (Purinergic P1 Receptor Agonists)
RN  - 138-81-8 (2,3-Diphosphoglycerate)
RN  - 1F7A44V6OU (Colforsin)
RN  - 24386-93-4 (5-iodotubercidin)
RN  - 50908-62-8 (N-cyclopropyl adenosine-5'-carboxamide)
RN  - 5YFR5SPS6T (3,7-dimethyl-1-propargylxanthine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - K72T3FS567 (Adenosine)
RN  - M351LCX45Y (Tubercidin)
RN  - OBD445WZ5P (Theobromine)
SB  - IM
MH  - 2,3-Diphosphoglycerate
MH  - Adenosine/analogs & derivatives/*pharmacology
MH  - Adenosine Deaminase Inhibitors
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Biological Transport
MH  - Carbonic Anhydrases/*blood
MH  - Chick Embryo
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*physiology
MH  - Diphosphoglyceric Acids/*blood
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Erythrocytes/*metabolism
MH  - Fetal Blood/*metabolism/physiology
MH  - Nucleosides/antagonists & inhibitors/metabolism
MH  - Protein Biosynthesis
MH  - Purinergic P1 Receptor Agonists
MH  - Theobromine/analogs & derivatives/pharmacology
MH  - Tubercidin/analogs & derivatives/pharmacology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1152/ajpregu.1996.271.4.R973 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Oct;271(4 Pt 2):R973-81. doi: 10.1152/ajpregu.1996.271.4.R973.