PMID- 8897991
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 271
IP  - 4 Pt 2
DP  - 1996 Oct
TI  - Norepinephrine-mediated hypoxic stimulation of embryonic red cell carbonic 
      anhydrase and 2,3-DPG synthesis.
PG  - R982-9
AB  - Hypoxia is the stimulus for activation of red cell carbonic anhydrase II (CAII) 
      and 2,3-diphosphoglycerate (2,3-DPG) synthesis of chick red blood cells during 
      late embryonic development. We have tested whether plasma catecholamines are 
      involved as hormonal mediators, because hypoxia is a well-known stimulus for 
      catecholamine release in mammalian fetuses. Plasma catecholamines were measured 
      in 8- to 16-day-old chick embryos. Plasma levels of norepinephrine (NE) were 
      initially low, but its concentration increased rapidly from 2.7 nM (day 12) to 
      13.4 nM at day 13 and 25.5 nM at day 16. Epinephrine (E) was not detectable 
      before day 13. Short-term hypoxic exposure of day 11 embryos (1-h incubation at 
      13.5% O2) increased plasma NE concentration fivefold compared with the controls 
      but had no effect on E. During 15-h in vitro incubation of red blood cells from 
      day 11, addition of 1 microM NE to the incubation medium increased the red cell 
      2,3-DPG concentration nearly threefold and CAII activity sixfold compared with 
      the control. The CAII activity and 2,3-DPG concentration were also increased when 
      cells were incubated with plasma from late chick embryos. The activation was 
      induced by beta-adrenergic stimulation of adenylyl cyclase. Atenolol and 
      propranolol blocked the effects of NE and embryonic chick plasma. Analysis of de 
      novo protein synthesis ([35S]methionine incorporation) demonstrated that 
      catecholamines stimulate the synthesis of several proteins besides CAII. The 
      results indicate that developmental changes of plasma NE concentration are 
      instrumental in the adenosine 3',5'-cyclic monophosphate-dependent activation of 
      CAII and 2,3-DPG synthesis of red blood cells from late chick embryos.
FAU - Dragon, S
AU  - Dragon S
AD  - Physiologisches Institut, Universitat Regensburg, Germany.
FAU - Glombitza, S
AU  - Glombitza S
FAU - Gotz, R
AU  - Gotz R
FAU - Baumann, R
AU  - Baumann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Diphosphoglyceric Acids)
RN  - 138-81-8 (2,3-Diphosphoglycerate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - 2,3-Diphosphoglycerate
MH  - Animals
MH  - Carbonic Anhydrases/*blood
MH  - Chick Embryo
MH  - Cyclic AMP/blood
MH  - Diphosphoglyceric Acids/*blood
MH  - Epinephrine/blood/pharmacology
MH  - Fetal Blood/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Hypoxia/*blood
MH  - Norepinephrine/blood/pharmacology/*physiology
MH  - Osmolar Concentration
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1152/ajpregu.1996.271.4.R982 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Oct;271(4 Pt 2):R982-9. doi: 10.1152/ajpregu.1996.271.4.R982.