PMID- 8898953
OWN - NLM
STAT- MEDLINE
DCOM- 19961210
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 26
IP  - 10
DP  - 1996 Oct
TI  - Expression and cleavage of tumor necrosis factor-alpha and tumor necrosis factor 
      receptors by human monocytic cell lines upon direct contact with stimulated T 
      cells.
PG  - 2404-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a potent cytokine in inflammatory 
      processes. A variety of mechanisms that modulate its activity have been 
      described, one being its binding to soluble receptors (sTNFR). In this study, we 
      demonstrate that human monocytic cells such as THP-1 respond to direct contact 
      with a membrane preparation of stimulated HUT-78 cells by producing TNF-alpha and 
      by releasing sTNFR-p75, but not sTNFR-p55, with different kinetics. TNF-alpha 
      concentration peaked after 12 h of contact and then decreased, whereas sTNFR-p75 
      production increased progressively upon cell/cell contact. The decrease in 
      TNF-alpha concentration is not due to trapping of TNF-alpha by its soluble 
      receptors or other soluble or cell-associated molecules, but rather to a 
      proteolytic activity associated to THP-1 cells. On the other hand, the increase 
      in sTNFR-p75 release does not result from an increase in the cleavage of 
      pre-existing cell-associated sTNFR-p75 but from an increase in TNFR-p75 
      expression, immediately followed by the cleavage of its extracellular domain. 
      Phenylmethylsulfonylfluoride, a serine protease inhibitor, has a negative effect 
      on both TNF-alpha degradation and sTNFR-p75 release by THP-1 cells. Thus, there 
      may be an enzymatic activity associated to THP-1 cells that plays an important 
      role in the neutralization of TNF-alpha activity both by degrading the molecule 
      and by cleaving its receptors at the cell surface.
FAU - Vey, E
AU  - Vey E
AD  - Department of Internal Medicine, Hopital Cantonal Universitaire, Geneva, 
      Switzerland
FAU - Burger, D
AU  - Burger D
FAU - Dayer, J M
AU  - Dayer JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Humans
MH  - Lymphocyte Activation
MH  - Monocytes/immunology/*metabolism
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Serine Endopeptidases/metabolism
MH  - Signal Transduction
MH  - Solubility
MH  - T-Lymphocytes/*immunology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1002/eji.1830261021 [doi]
PST - ppublish
SO  - Eur J Immunol. 1996 Oct;26(10):2404-9. doi: 10.1002/eji.1830261021.