PMID- 8901921
OWN - NLM
STAT- MEDLINE
DCOM- 19961206
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 113
IP  - 1-3
DP  - 1996 Oct 28
TI  - Cytogenetic effects of butadiene metabolites in rat and mouse splenocytes 
      following in vitro exposures.
PG  - 336-40
AB  - As a first step in investigating the genotoxic effects of the principal 
      metabolites of 1,3-butadiene (BD) in both rats and mice, splenocytes (which have 
      little mixed function oxidase activity) from each specimen were exposed to a 
      series of concentrations of either 3,4-epoxy-1-butene (EB) (20 to 931 microM) or 
      1,2:3,4-diepoxybutane (DEB) (2.5 to 160 microM) for 1 h. The splenocytes were 
      then washed, cultured, and stimulated to divide with concanavalin A, and 
      metaphases were analyzed for the induction of sister chromatid exchanges (SCEs) 
      and chromosome aberrations (CAs). In addition, cells from some experiments were 
      taken after exposure but before culture, and subjected to the single cell gel 
      (SCG) assay to measure DNA damage in the form of DNA strand breakage and/or 
      alkaline-labile sites. Initial studies indicate that EB does not induce 
      cytogenetic damage in either rat or mouse G0 splenocytes. However, DEB was an 
      extremely potent SCE- and CA-inducer in both species with no species differences 
      apparent. Neither DEB nor EB produced any statistically significant DNA-damaging 
      effects as measured by the SCG assay.
FAU - Kligerman, A D
AU  - Kligerman AD
AD  - U.S. Environmental Protection Agency, Environmental Carcinogenesis Division, 
      Research Triangle Park, NC 27711, USA.
FAU - Doerr, C L
AU  - Doerr CL
FAU - Milholland, V S
AU  - Milholland VS
FAU - Tennant, A H
AU  - Tennant AH
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Epoxy Compounds)
RN  - 0 (Mutagens)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - 60OB65YNAB (diepoxybutane)
SB  - IM
MH  - Animals
MH  - Cell Cycle/drug effects
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Epoxy Compounds/*toxicity
MH  - Male
MH  - Mice
MH  - Mutagens/*toxicity
MH  - Rats
MH  - *Sister Chromatid Exchange
MH  - Species Specificity
MH  - Spleen/cytology/drug effects
EDAT- 1996/10/28 00:00
MHDA- 1996/10/28 00:01
CRDT- 1996/10/28 00:00
PHST- 1996/10/28 00:00 [pubmed]
PHST- 1996/10/28 00:01 [medline]
PHST- 1996/10/28 00:00 [entrez]
AID - 0300-483X(96)03469-5 [pii]
AID - 10.1016/0300-483x(96)03469-5 [doi]
PST - ppublish
SO  - Toxicology. 1996 Oct 28;113(1-3):336-40. doi: 10.1016/0300-483x(96)03469-5.