PMID- 8902213
OWN - NLM
STAT- MEDLINE
DCOM- 19970207
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 55
IP  - 5
DP  - 1996 Nov
TI  - Zonal distribution of 11 beta-hydroxysteroid dehydrogenase types 1 and 2 
      messenger ribonucleic acid expression in the rat placenta and decidua during late 
      pregnancy.
PG  - 1023-8
AB  - Glucocorticoid action in several target tissues is dependent on expression of 11 
      beta-hydroxysteroid dehydrogenase (11 beta-HSD), and in the placenta 11 beta-HSD 
      is thought to regulate transfer of active glucocorticoid to the fetus. This study 
      compared expression of the two recognized 11 beta-HSD enzymes, types 1 and 2, in 
      the rat placenta and decidua on Days 16 and 22 of gestation (term = Day 23). 
      According to S1 nuclease protection analysis, although mRNA for 11 beta-HSD-1 was 
      only just detectable in the labyrinth zone on Day 16, by Day 22 this expression 
      had increased almost 20-fold. There was also an increase (approximately 2-fold) 
      in 11 beta-HSD-1 mRNA in the basal zone between Days 16 and 22. In Day 16 
      decidua, 11 beta-HSD-1 mRNA was also highly expressed, but insufficient tissue 
      was available for analysis on Day 22. Western blot analysis showed that 
      immunoreactive 11 beta-HSD-1 (molecular mass 34 kDa) was present in those tissues 
      with the highest 11 beta-HSD-1 mRNA expression (Day 16 decidua and Day 22 
      labyrinth zone). With respect to mRNA for 11 beta-HSD-2, high expression was 
      observed in the decidua and labyrinth zone at Day 16, but in the latter this 
      expression then declined 90% by Day 22. In contrast, expression of mRNA for 11 
      beta-HSD-2 increased more than 3-fold in the basal zone over the same period. 
      Consistent with coexpression of the two 11 beta-HSD enzymes, both 11-oxoreductase 
      and 11 beta-dehydrogenase bioactivity were clearly evident in all tissues, and 
      each varied with stage of gestation. Specifically, 11 beta-dehydrogenase activity 
      in the basal zone increased from 38 +/- 2% (mean +/- SEM) on Day 16 to 56 +/- 2% 
      on Day 22, while 11-oxoreductase activity fell from 55 +/- 3% to 43 +/- 2% over 
      the same period. In contrast, 11 beta-dehydrogenase activity in the labyrinth 
      zone fell with advancing pregnancy (Day 16: 63 +/- 2%; Day 22: 48 +/- 2%). Both 
      11-oxoreductase (58 +/- 3%) and 11 beta-dehydrogenase (38 +/- 4%) activities were 
      also evident in decidua at Day 16. In conclusion, this study shows that 
      expression of 11 beta-HSD-1 and -2 is zone-specific in the placenta and maternal 
      decidua. Moreover, opposite changes in the expression of the two enzymes occur in 
      the basal and labyrinth zones of the placenta over the last days of pregnancy, 
      indicative of distinct regulatory mechanisms and functional significance for the 
      enzymes in the two placental zones.
FAU - Burton, P J
AU  - Burton PJ
AD  - Department of Anatomy and Human Biology, University of Western Australia, 
      Nedlands, Perth, Australia.
FAU - Smith, R E
AU  - Smith RE
FAU - Krozowski, Z S
AU  - Krozowski ZS
FAU - Waddell, B J
AU  - Waddell BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Blotting, Western
MH  - Decidua/*enzymology
MH  - Female
MH  - Hydroxysteroid Dehydrogenases/*genetics
MH  - Placenta/*enzymology
MH  - Pregnancy
MH  - RNA, Messenger/*analysis
MH  - Rats
MH  - Rats, Wistar
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1095/biolreprod55.5.1023 [doi]
PST - ppublish
SO  - Biol Reprod. 1996 Nov;55(5):1023-8. doi: 10.1095/biolreprod55.5.1023.