PMID- 8902469
OWN - NLM
STAT- MEDLINE
DCOM- 19970213
LR  - 20190822
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 9
IP  - 10
DP  - 1996 Oct
TI  - Defective inhibition of sodium on basophil histamine release in patients with 
      allergic rhinitis and bronchial asthma.
PG  - 2070-6
AB  - The aim of this study was to evaluate whether Na+ exerts its inhibitory effect on 
      basophil histamine release induced by immunoglobulin E (IgE)-dependent (anti-IgE) 
      and IgE-independent (N-formyl-methionyl-leucyl-phenylalanine (FMLP), 
      interleukin-3 (IL-3)) stimuli in patients with allergic rhinitis (n = 24) and 
      allergic bronchial asthma (n = 10). Peripheral blood leucocytes were stimulated 
      with anti-IgE, FMLP and IL-3 in the presence of high and low Na+ concentrations, 
      and histamine release was measured using a fluorometric method. In standard 
      Na(+)-containing medium, spontaneous and stimulated histamine release was higher 
      in allergic patients (n = 34) (both rhinitic and asthmatic) than in healthy 
      subjects (n = 41). Na+ removal from extracellular medium and its isosmotic 
      substitution with choline chloride or with N-methyl-D-glucamine led to a 
      significant increase of anti-IgE-, FMLP- and IL-3-induced histamine release in 
      normal subjects, but not in allergic patients. The increase in Na+ concentration 
      in the extra-cellular medium was accompanied by a dose-dependent decrease of 
      anti-IgE- and FMLP-induced histamine release in normal subjects, but not in 
      allergic patients. The behaviour of atopics and healthy subjects was different 
      and not related to the basophil responsiveness to activating signals. The 
      incubation of basophils from healthy subjects with sera from allergic patients 
      did not have a significant influence on the inhibitory effect of Na+. Basophils 
      from healthy subjects and atopic patients respond differently when stimulated in 
      a low Na+ medium. The reduced sensitivity to the inhibitory effect of Na+ may 
      contribute to basophil dysfunction in patients with respiratory allergy.
FAU - Tedeschi, A
AU  - Tedeschi A
AD  - Dept of Internal Medicine, University of Milan, IRCCS Ospedale Maggiore 
      Policlinico, Italy.
FAU - Cottini, M
AU  - Cottini M
FAU - Salmaso, C
AU  - Salmaso C
FAU - Milazzo, N
AU  - Milazzo N
FAU - Miadonna, A
AU  - Miadonna A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Chlorides)
RN  - 0 (Interleukin-3)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 6HG8UB2MUY (Meglumine)
RN  - 9NEZ333N27 (Sodium)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Adult
MH  - Asthma/immunology/*metabolism
MH  - Basophils/*drug effects/immunology/metabolism
MH  - Chlorides/pharmacology
MH  - Choline/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Extracellular Space
MH  - Female
MH  - Fluorometry
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - Hypersensitivity, Immediate/immunology/metabolism
MH  - Immunoglobulin E/immunology
MH  - Interleukin-3/immunology
MH  - Leukocytes/drug effects/immunology
MH  - Male
MH  - Meglumine/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/immunology
MH  - Osmosis
MH  - Respiratory Hypersensitivity/immunology/metabolism
MH  - Rhinitis, Allergic, Perennial/immunology/*metabolism
MH  - Rhinitis, Allergic, Seasonal/immunology/*metabolism
MH  - Sodium/*pharmacology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1183/09031936.96.09102070 [doi]
PST - ppublish
SO  - Eur Respir J. 1996 Oct;9(10):2070-6. doi: 10.1183/09031936.96.09102070.