PMID- 8902521
OWN - NLM
STAT- MEDLINE
DCOM- 19970218
LR  - 20191210
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 21
IP  - 6
DP  - 1996
TI  - The effects of alpha-phenyl-tert-butyl nitrone (PBN) on copper-induced rat 
      fulminant hepatitis with jaundice.
PG  - 755-61
AB  - In the present study we demonstrated the protective effects of the spin-trapping 
      agent alpha-phenyl-tert-butyl nitrone (PBN) against fulminant hepatitis with 
      jaundice in LEC rats. In LEC rats an excess amount of copper is accumulated in 
      the liver and causes hepatitis with severe jaundice. PBN was subcutaneously 
      administered every 2 d at the concentration of 128 mg/kg, beginning with 
      13-week-old rats and continuing for 17 weeks. PBN prevented the loss of body 
      weight, reduced death rate, and suppressed the increase in GTP and GOT values 
      reflecting hepatic cell destruction. Ocular inspection also confirmed the 
      suppressive effects of PBN on jaundice. In parallel with these phenomena, the 
      amounts of thiobarbituric acid-reactive substances (TBARS) in livers of 
      PBN-administered rats were found to be lower than those of non-PBN-administered 
      rats. Little histological changes were observed in PBN-administered rats in 
      comparison with non-PBN-administered rats. The protective effect of PBN on the 
      formation of oxidative damage in liver DNA was observed but not so remarkable as 
      that on lipid peroxidation. From these results, it was concluded that PBN had the 
      liver-protective effects against fulminant hepatitis with jaundice. This 
      suggested that free radicals play an important role in abnormally accumulated 
      copper-induced liver injury and that PBN potentially has therapeutic value for 
      the treatment of hepatitis.
FAU - Yamashita, T
AU  - Yamashita T
AD  - Department of Animal Disease Control, Laboratory of Radiation Biology, Graduate 
      School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Ohshima, H
AU  - Ohshima H
FAU - Asanuma, T
AU  - Asanuma T
FAU - Inukai, N
AU  - Inukai N
FAU - Miyoshi, I
AU  - Miyoshi I
FAU - Kasai, N
AU  - Kasai N
FAU - Kon, Y
AU  - Kon Y
FAU - Watanabe, T
AU  - Watanabe T
FAU - Sato, F
AU  - Sato F
FAU - Kuwabara, M
AU  - Kuwabara M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Cyclic N-Oxides)
RN  - 0 (Nitrogen Oxides)
RN  - 0 (Spin Labels)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 3I91332OPG (phenyl-N-tert-butylnitrone)
RN  - 789U1901C5 (Copper)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - 9007-49-2 (DNA)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Aging
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Aspartate Aminotransferases/blood
MH  - Chemical and Drug Induced Liver Injury/*prevention & control
MH  - *Copper
MH  - Cyclic N-Oxides
MH  - DNA/metabolism
MH  - Deoxyguanosine/analogs & derivatives/metabolism
MH  - Jaundice/chemically induced/*prevention & control
MH  - Lipid Peroxidation
MH  - Liver/metabolism
MH  - Nitrogen Oxides/*therapeutic use
MH  - Rats
MH  - Rats, Mutant Strains
MH  - *Spin Labels
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Weight Loss
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0891584996002225 [pii]
AID - 10.1016/0891-5849(96)00222-5 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 1996;21(6):755-61. doi: 10.1016/0891-5849(96)00222-5.