PMID- 8903391
OWN - NLM
STAT- MEDLINE
DCOM- 19961212
LR  - 20071115
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 24
IP  - 5
DP  - 1996 Nov
TI  - Chemokine levels in human liver homogenates: associations between GRO alpha and 
      histopathological evidence of alcoholic hepatitis.
PG  - 1156-60
AB  - Alcoholic hepatitis is characterized by parenchymal neutrophil infiltration. 
      Hepatic synthesis of the neutrophil chemokine interleukin-8 (IL-8) is highly 
      elevated in alcoholic hepatitis and levels correlate with the degree of 
      neutrophil infiltration. The aim of this study was to further determine the 
      spectrum of synthesis of chemokines in liver tissue from patients with alcoholic 
      liver disease and a range of disease control subjects. Subjects were composed of 
      24 patients with alcoholic liver disease of whom 15 had histopathological 
      evidence of alcoholic hepatitis (10 cirrhotic) and 9 no evidence of alcoholic 
      hepatitis (5 cirrhotic); other controls included; normal liver (n = 6), viral 
      hepatitis (n = 16), primary biliary cirrhosis (n = 5), acute liver failure (n = 
      4), and miscellaneous liver disease (n = 13). Levels of the C-X-C neutrophil 
      chemokine GRO alpha and the mononuclear cell C-C chemokines: macrophage 
      inflammatory protein 1 alpha, macrophage chemotactic protein 1 and RANTES, were 
      determined by ELISA in liver homogenates. Levels of the neutrophil chemokine GRO 
      alpha were specifically elevated (mean 46 pg/mg, compared with normal liver 11 
      pg/mg) in patients with alcoholic hepatitis. GRO alpha levels correlated with 
      IL-8 levels and were higher in patients with alcoholic liver disease and 
      parenchymal neutrophil infiltration. Hepatic RANTES was elevated in diseased 
      liver, with the highest levels found in viral hepatitis (mean 117 pg/mg, compared 
      with 24 pg/mg in normal liver). No significant changes in hepatic levels of 
      macrophage inflammatory protein 1 alpha (MIP-1 alpha) or macrophage chemotactic 
      protein 1 (MCP-1) were found. These data provide further supportive evidence that 
      parenchymal neutrophil infiltration in alcoholic hepatitis may be determined by 
      selective upregulation of C-X-C chemokine synthesis.
FAU - Maltby, J
AU  - Maltby J
AD  - Department of Medicine, University of Southampton, Southampton General Hospital, 
      UK.
FAU - Wright, S
AU  - Wright S
FAU - Bird, G
AU  - Bird G
FAU - Sheron, N
AU  - Sheron N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Chemokine CCL2/analysis
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/analysis
MH  - Chemokine CXCL1
MH  - Chemokines/*analysis
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*analysis
MH  - Female
MH  - Growth Substances/*analysis
MH  - Hepatitis, Alcoholic/metabolism/*pathology
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Liver/*chemistry
MH  - Macrophage Inflammatory Proteins/analysis
MH  - Male
MH  - Middle Aged
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S0270-9139(96)00470-3 [pii]
AID - 10.1053/jhep.1996.v24.pm0008903391 [doi]
PST - ppublish
SO  - Hepatology. 1996 Nov;24(5):1156-60. doi: 10.1053/jhep.1996.v24.pm0008903391.