PMID- 8904436
OWN - NLM
STAT- MEDLINE
DCOM- 19970225
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 34
IP  - 3
DP  - 1996 Mar
TI  - Evaluation of a commercial enzyme-linked immunosorbent assay for detection of 
      serum immunoglobulin G response to human herpesvirus 6.
PG  - 675-9
AB  - A rapid (60-min) commercially available enzyme-linked immunosorbent assay (ELISA) 
      for the detection of immunoglobulin G (IgG) class antibodies to human herpesvirus 
      6 (HHV-6) was evaluated. The specificity of the ELISA for HHV-6 was confirmed by 
      absorption studies, with the reactivities of HHV-6-positive sera being unaffected 
      by other herpesviruses (cytomegalovirus, herpes simplex virus, and 
      varicella-zoster virus) or the HSB2 cell line used to culture HHV-6. HHV-6 IgG 
      antibody levels in a panel of 502 serum samples were determined by ELISA and an 
      indirect immunofluorescence assay (IFA). Results obtained by the two methods were 
      in close agreement, suggesting that the ELISA provides a suitable test method for 
      the determination of HHV-6 IgG antibodies in a routine clinical laboratory. Both 
      tests were positive in 398 cases (79%), and both were negative in 71 cases (14%), 
      with a different result obtained by IFA and ELISA in only 33 cases (7%). 
      Furthermore, absorption of sera with HHV-6 prior to assay revealed that the 
      majority of these results were false positive (n = 8) or false negative (n = 23) 
      in the IFA (true positives or negatives in the ELISA). Subsequently, the ELISA 
      showed a sensitivity of 99.76% and a specificity of 98.75%. HHV-6-specific IgG 
      levels were also determined in paired serum samples collected from 49 donors--14 
      with exanthem subitum (ES), 15 with ES which was complicated with central nervous 
      system involvement, and 20 undergoing bone marrow transplantation--in whom HHV-6 
      infection had been demonstrated by virus isolation and/or PCR. All patients with 
      ES or central nervous system complications showed an increase in HHV-6-specific 
      IgG, indicating that this ELISA may be a useful aid in the diagnosis of these 
      conditions. Furthermore, 14 of 20 patients undergoing bone marrow transplantation 
      showed an increase in HHV-6-specific IgG levels, possibly reflecting a 
      reactivation of HHV-6 in these patients.
FAU - Sloots, T P
AU  - Sloots TP
AD  - Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, 
      Royal Children's Hospital, Brisbane, Australia.
FAU - Kapeleris, J P
AU  - Kapeleris JP
FAU - Mackay, I M
AU  - Mackay IM
FAU - Batham, M
AU  - Batham M
FAU - Devine, P L
AU  - Devine PL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/*blood
MH  - Bone Marrow Transplantation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Immunoglobulin G/*blood
MH  - Middle Aged
PMC - PMC228868
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
PMCR- 1996/03/01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PHST- 1996/03/01 00:00 [pmc-release]
AID - 10.1128/jcm.34.3.675-679.1996 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1996 Mar;34(3):675-9. doi: 10.1128/jcm.34.3.675-679.1996.