PMID- 8904877
OWN - NLM
STAT- MEDLINE
DCOM- 19961224
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 95
IP  - 2
DP  - 1996 Nov
TI  - Inhibition of tissue factor surface expression in human peripheral blood 
      monocytes exposed to cytokines.
PG  - 249-57
AB  - Interleukin (IL)-4, IL-10, IL-13 and transforming growth factor beta (TGF-beta) 
      are known to regulate several monocyte functions, including inhibition of the 
      synthesis of different cytokines. Using quantitative RT-PCR and flow cytometry 
      analysis we investigated the effects of these cytokines on bacterial 
      lipopolysaccharide (LPS)-induced tissue factor (TF) expression in human 
      monocytes. The effects of IL-4 and IL-10 on monocyte chemoattractant protein-1 
      (MCP-1)-and C-reactive protein (CRP)-induced TF expression were also studied. A 
      direct comparison revealed that IL-4, IL-10 and IL-13 all down-regulated 
      LPS-induced TF expression in a concentration-dependent manner without the need 
      for priming. In contrast, TGF-beta required 4 h of priming to inhibit TF 
      expression induced by LPS. IL-10 was the most powerful inhibitor, causing almost 
      complete inhibition at 5 ng/ml. IL-4 and IL-13 exhibited a significantly lower 
      inhibitory capacity even at concentrations of 100 ng/ml. IL-4 and IL-10 showed 
      similar concentration-dependent inhibition of MCP-1- and CRP-induced TF 
      expression. We also showed that the regulatory effect of the interleukins 
      occurred at the mRNA level. In vivo, these inhibitory cytokines may play an 
      important regulatory role in preventing thrombosis. IL-10, in particular, may be 
      a possible candidate as a TF-preventing drug.
FAU - Ernofsson, M
AU  - Ernofsson M
AD  - Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
FAU - Tenno, T
AU  - Tenno T
FAU - Siegbahn, A
AU  - Siegbahn A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Interleukin-13)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - C-Reactive Protein/pharmacology
MH  - Humans
MH  - Interleukin-10/*pharmacology
MH  - Interleukin-13/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Thromboplastin/*antagonists & inhibitors
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1996.d01-1893.x [doi]
PST - ppublish
SO  - Br J Haematol. 1996 Nov;95(2):249-57. doi: 10.1046/j.1365-2141.1996.d01-1893.x.