PMID- 8905407
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 52
IP  - 2-3
DP  - 1996 Sep
TI  - IgE antibody up-regulates high affinity IgE binding on murine bone marrow-derived 
      mast cells.
PG  - 129-34
AB  - We have examined 3-week-old alcian blue positive cells (putatively mast cells) 
      derived from mouse bone marrow for their expression of Fc epsilon RI. Using an 
      indirect method of sensitizing the cells with immunoglobulin E (IgE) antibody 
      (anti-DNP IgE) and detecting the level of bound IgE antibody by flow cytometry, 
      we found that prolonged culture (1-5 days) with IgE, but not IgG, increased the 
      total receptor density 6 +/- 1.9 fold. During the same period, histamine release 
      in response to antigen (DNP-HSA) increased approximately 6-fold while the cell's 
      response to either thrombin or ionomycin remained constant. The greatest 
      up-regulation occurred in the first 2 days of culture. Using 2.4G2 to detect Fc 
      epsilon RII RIII, we could not detect any up-regulation of this receptor. 
      Culturing the cells for 1 h after sensitization did not result in any loss of 
      cell surface IgE, suggesting a reasonably high affinity binding similar to that 
      expected for Fc epsilon RI. This up-regulation was completely inhibited by 
      co-culture with 2 micrograms/ml cycloheximide. These data suggest that IgE is 
      capable of inducing a significant, protein synthesis, dependent up-regulation of 
      its own high affinity receptor on mast cells/basophils.
FAU - Hsu, C
AU  - Hsu C
AD  - Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
FAU - MacGlashan, D Jr
AU  - MacGlashan D Jr
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Animals
MH  - Bone Marrow/immunology
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - Cycloheximide/pharmacology
MH  - Dinitrobenzenes/immunology
MH  - Histamine Release
MH  - Immunoglobulin E/*metabolism
MH  - Mast Cells/drug effects/*immunology
MH  - Mice
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Receptors, IgE/*metabolism
MH  - Up-Regulation
EDAT- 1996/09/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 0165-2478(96)02599-0 [pii]
AID - 10.1016/0165-2478(96)02599-0 [doi]
PST - ppublish
SO  - Immunol Lett. 1996 Sep;52(2-3):129-34. doi: 10.1016/0165-2478(96)02599-0.