PMID- 8908163
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 91
IP  - 1
DP  - 1996 Oct 1
TI  - Molecular cytogenetic analysis of five newly established cervical cancer cell 
      lines using G banding and fluorescence in situ hybridization.
PG  - 28-36
AB  - Cervical tumors nearly all have complex karyotypes and more precise cytogenetic 
      information is required to establish whether specific rearrangements occur, and 
      if they are related to the type of HPV infection found. The karyotypes of five 
      recently established cervical cancer cell lines, three from squamous cell 
      carcinomas (two HPV 16 +ve and one HPV 18 +ve), one from an adenocarcinoma (HPV 
      -ve), and one from an adenosquamous carcinoma (HPV 16 +ve), have been analysed 
      using fluorescence in situ hybridization (FISH), with 23 chromosome specific 
      paints, YACs and cosmids as probes, in addition to conventional G banding, in 
      order to identify markers and clarify the breakpoints. Chromosomes 1 and 3 were 
      rearranged in all cell lines. Breakpoints in the squamous lines were all in 3q. 
      but in different regions. Small metacentrics involving chromosome 5 were a 
      del(5q) in one line, and a t(X;5) in another, rather than i(5p). The region 6q21 
      was involved in three cases and chromosome 9 was rearranged in four. An i(8q) was 
      found in three squamous carcinoma cell lines. Structural changes of 11q were 
      found only in two cases, but a marker 11 representing amplification in the 
      11q14-22 region was duplicated in the adenosquamous line.
FAU - Thein, A T
AU  - Thein AT
AD  - Department of Genetics & Biometry, UCL, London, UK.
FAU - Han, X
AU  - Han X
FAU - Heyderman, E
AU  - Heyderman E
FAU - Fox, M
AU  - Fox M
FAU - Steele, S J
AU  - Steele SJ
FAU - Parrington, J M
AU  - Parrington JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - 3T3 Cells
MH  - Adult
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Banding
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mice
MH  - Middle Aged
MH  - *Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*genetics
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0165460896001100 [pii]
AID - 10.1016/s0165-4608(96)00110-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Oct 1;91(1):28-36. doi: 
      10.1016/s0165-4608(96)00110-0.