PMID- 8908165
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 91
IP  - 1
DP  - 1996 Oct 1
TI  - Complex chromosome translocations of standard t(8;21) and t(15;17) arise from a 
      two-step mechanism as evidenced by fluorescence in situ hybridization analysis.
PG  - 40-5
AB  - The authors report the results of cytogenetic and fluorescence in situ 
      hybridization (FISH) analysis performed on complex chromosome translocations 
      (CCTs) of t(8;21) and t(15;17) standard translocations associated with two M2 
      subtypes of acute myeloid leukemia (AML-M2) and four acute promyelocytic leukemia 
      (APL), respectively. In one of two AML-M2 patients FISH analysis showed part of 
      chromosome 21 on the der(8) and material from this chromosome on the der(21) and 
      on chromosome 1 at band p32, suggesting that the t(8;21) occurred as the primary 
      step. In the second AML-M2 patient. FISH displayed part of chromosome 21 on the 
      der(8) and material from this chromosome on the der(21) but not on the third 
      rearranged chromosome. Therefore, it is unclear whether chromosome 2 was 
      rearranged secondary to the standard t(8;21). In four APL patients, FISH analysis 
      showed material derived from chromosome 17 on the der(15). Moreover, in two 
      patients with an i(17q) FISH disclosed material from chromosome 15 at the ends of 
      both arms of the i(17q), suggesting that it occurred after the standard t(15;17). 
      In the remaining two APL patients, FISH showed material from chromosome 15 on the 
      der(17) and on chromosome 21 at band q22 in one case, and material of the p arm 
      of chromosome 17 on chromosome 4 at band q11 in the other, demonstrating that in 
      these two cases the first mutation also had been the t(15;17). Therefore, FISH 
      analysis revealed that CCTs in five patients were secondary changes which 
      occurred after standard t(8;21) and t(15;17), thus clarifying the hierarchy of 
      the cytogenetic events, their role in the pathogenesis of the disease, and the 
      associated clinic-hematologic findings.
FAU - Calabrese, G
AU  - Calabrese G
AD  - Istituto di Biologia e Genetica, Universita di Chieti, Italy.
FAU - Min, T
AU  - Min T
FAU - Stuppia, L
AU  - Stuppia L
FAU - Powles, R
AU  - Powles R
FAU - Swansbury, J G
AU  - Swansbury JG
FAU - Morizio, E
AU  - Morizio E
FAU - Peila, R
AU  - Peila R
FAU - Donti, E
AU  - Donti E
FAU - Fioritoni, G
AU  - Fioritoni G
FAU - Palka, G
AU  - Palka G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Leukemia, Promyelocytic, Acute/*genetics
MH  - Male
MH  - Translocation, Genetic/*genetics
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0165460896000969 [pii]
AID - 10.1016/s0165-4608(96)00096-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Oct 1;91(1):40-5. doi: 
      10.1016/s0165-4608(96)00096-9.