PMID- 8908166
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 91
IP  - 1
DP  - 1996 Oct 1
TI  - Epithelioid sarcoma with an 18q aberration.
PG  - 46-52
AB  - Epithelioid sarcoma is a peculiar soft-tissue neoplasm of uncertain origin, which 
      is characterized by an epithelioid morphology of tumor cells coexpressing 
      epithelial (keratin) and nonepithelial (vimentin) antigens. We herein report a 
      new cytogenetic abnormality with der(22)t(18;22)(q11;p11.2) in a case of 
      epithelioid sarcoma that occurred in the elbow of a 75-year-old man. 
      Histologically, the tumor demonstrated a multinodular proliferation of 
      epithelioid cells, with positive immunostaining for keratin, epithelial membrane 
      antigen (EMA), and vimentin. Cultured tumor cells obtained from fresh surgical 
      materials were frozen in plastic ampules and stocked in a liquid nitrogen 
      freezer. Six years after surgery, the cells were recovered from the freezer and 
      utilized for both morphologic and cytogenetic analyses. These cultured cells both 
      before and after the freezing exhibited essentially the same epithelioid 
      morphology and immunophenotypes as those of the original tumor. A chromosome 
      analysis, together with fluorescence in situ hybridization (FISH), demonstrated a 
      61-67 modal population, and a characteristic clonal abnormality with 
      der(22)t(18;22)(q11;p11.2). Other clonal abnormalities included numerical (-3, 
      -4, +7, -13, -14, -16, -18, +20, -22) and structural (8p+, 9p+, 12p+, i(21q)) 
      aberrations. Some variant clones also demonstrated i(18q). Since the breakpoint 
      at 18q11 is similar to that reported in synovial sarcoma, this finding may 
      support the presence of a histogenetic relationship between epithelioid sarcoma 
      and synovial sarcoma. Our study thus indicates that the storage of frozen cells 
      is useful for both morphologic and cytogenetic analyses of soft tissue tumors.
FAU - Iwasaki, H
AU  - Iwasaki H
AD  - Departments of Pathology, Fukuoka University School of Medicine, Japan.
FAU - Ohjimi, Y
AU  - Ohjimi Y
FAU - Ishiguro, M
AU  - Ishiguro M
FAU - Isayama, T
AU  - Isayama T
FAU - Kaneko, Y
AU  - Kaneko Y
FAU - Yoh, S
AU  - Yoh S
FAU - Emoto, G
AU  - Emoto G
FAU - Kikuchi, M
AU  - Kikuchi M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Elbow
MH  - Fatal Outcome
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Sarcoma/*genetics/pathology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0165460895003150 [pii]
AID - 10.1016/s0165-4608(95)00315-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Oct 1;91(1):46-52. doi: 
      10.1016/s0165-4608(95)00315-0.