PMID- 8912654
OWN - NLM
STAT- MEDLINE
DCOM- 19961213
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 228
IP  - 1
DP  - 1996 Nov 1
TI  - Evidence for reduced capacity for Ca2+ removal from the cytosol in submandibular 
      cells of newborn rats.
PG  - 171-5
AB  - The increase in cytosolic free Ca2+ concentration ([Ca2+]i) seen in submandibular 
      cells of early postnatal rats following exposure to acetylcholine (ACh) is larger 
      than in cells of adult rats. To elucidate possible reasons for this difference, 
      we compared Ca2+ movements through Ca2+ pumps in both types of cells using 
      Ca(2+)-sensitive fluorescent probe fura-2 and the radiotracer 45Ca2+. Ca2+ 
      release induced by endoplasmic reticulum (ER) Ca(2+)-pump inhibitor thapsigargin 
      (TG) was significantly smaller in neonatal cells than in adult cells, whereas the 
      inositol 1,4,5-trisphosphate (IP3)-elicited Ca2+ release was comparable in both 
      cell types. This suggests that although the size of the IP3-sensitive Ca2+ pool 
      is adequate in immature cells, the activity of TG-sensitive Ca2+ pump in this 
      pool is lower. The activity of the plasma membrane (PM) Ca(2+)-pump, measured by 
      extrusion of 45Ca2+, was also significantly lower in immature cells. These 
      results indicate that both ER and PM Ca2+ pumps may be functionally 
      underdeveloped in immature cells, and that the enhanced increase of [Ca2+]i seen 
      in response to ACh in immature cells may be partially, if not completely, due to 
      a reduced capacity for removal of Ca2+ from the cytosol by active mechanisms.
FAU - Zhang, G H
AU  - Zhang GH
AD  - Department of Pediatrics, University of Texas Health Science Center at San 
      Antonio 78284, USA.
FAU - Wells, J
AU  - Wells J
FAU - Willis, S
AU  - Willis S
FAU - Martinez, J R
AU  - Martinez JR
LA  - eng
GR  - DE04897/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - N9YNS0M02X (Acetylcholine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Cell Membrane/metabolism
MH  - Cytosol/metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - Female
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Submandibular Gland/*metabolism
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S0006-291X(96)91634-4 [pii]
AID - 10.1006/bbrc.1996.1634 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Nov 1;228(1):171-5. doi: 10.1006/bbrc.1996.1634.