PMID- 8912672
OWN - NLM
STAT- MEDLINE
DCOM- 19961226
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 319 ( Pt 2)
IP  - Pt 2
DP  - 1996 Oct 15
TI  - Lysophosphatidic acid-sensitive intracellular Ca2+ store does not regulate Ca2+ 
      entry at plasma membrane in Jurkat human T-cells.
PG  - 393-7
AB  - In Jurkat T cells, the anti-CD3 antibody OKT3 and thapsigargin (TG) elevated the 
      cytoplasmic free Ca2+ concentration ([Ca2+]i), after which it decreased to a 
      sustained, elevated level. In contrast, lysophosphatidic acid (LPA) increased 
      [Ca2+]i only briefly and transiently, after which it declined to the resting 
      level of [Ca2+]i even in the presence of extracellular Ca2+. OKT3 increased 
      Ins(1,4,5)P3 formation but neither LPA nor TG did. In the absence of 
      extracellular Ca2+, the addition of OKT3 did not affect an elevation of [Ca2+]i 
      induced by the subsequent addition of LPA and vice versa. In permeabilized Jurkat 
      cells, the addition of Ins(1,4,5)P3 released Ca2+; this was inhibited by heparin, 
      whereas LPA released Ca2+ even in the presence of heparin. cADP-ribose released 
      Ca2+; this was additive with LPA-induced Ca2+ release and vice versa in 
      permeabilized Jurkat cells. LPA did not stimulate Ca2+ entry and 45Ca2+ uptake 
      but OKT3 and TG did. LPA, OKT3 and TG did not affect the sustained elevation of 
      [Ca2+]i induced by ionomycin. The present results suggest that at least three 
      kinds of intracellular Ca2+ stores, which are Ins(1,4,5)P3,-cADP-ribose- and 
      LPA-sensitive, exist in Jurkat T cells, and that the LPA-sensitive intracellular 
      Ca2+ store does not regulate Ca2+ entry at the plasma membrane.
FAU - Takemura, H
AU  - Takemura H
AD  - Department of Pharmacology, School of Medicine, Sapporo Medical University, 
      Japan.
FAU - Imoto, K
AU  - Imoto K
FAU - Sakano, S
AU  - Sakano S
FAU - Kaneko, M
AU  - Kaneko M
FAU - Ohshika, H
AU  - Ohshika H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (CD3 Complex)
RN  - 0 (Lysophospholipids)
RN  - 0 (Muromonab-CD3)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - CD3 Complex/metabolism
MH  - Calcium/*metabolism
MH  - Humans
MH  - Ion Transport/drug effects
MH  - Jurkat Cells
MH  - Lysophospholipids/*pharmacology
MH  - Muromonab-CD3/pharmacology
MH  - Signal Transduction/*drug effects
MH  - T-Lymphocytes/*metabolism
PMC - PMC1217781
EDAT- 1996/10/15 00:00
MHDA- 1996/10/15 00:01
PMCR- 1997/04/15
CRDT- 1996/10/15 00:00
PHST- 1996/10/15 00:00 [pubmed]
PHST- 1996/10/15 00:01 [medline]
PHST- 1996/10/15 00:00 [entrez]
PHST- 1997/04/15 00:00 [pmc-release]
AID - 10.1042/bj3190393 [doi]
PST - ppublish
SO  - Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):393-7. doi: 10.1042/bj3190393.