PMID- 8912883
OWN - NLM
STAT- MEDLINE
DCOM- 19961209
LR  - 20131121
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 173
IP  - 2
DP  - 1996 Nov 1
TI  - Elevation of intracellular cyclic AMP induces an anergic-like state in Th1 
      clones.
PG  - 246-51
AB  - Because elevated intracellular cAMP suppresses T cell receptor (TCR)-mediated 
      effector activity and/or proliferation in response to antigen but does not always 
      affect IL-2-stimulated proliferation, the effects of cAMP on a T lymphocyte 
      response to antigen resemble antigen-induced anergy. To test the hypothesis that 
      elevated cAMP induces anergy in T lymphocytes, we have precultured murine Th1 
      clones responsive to porcine myelin basic protein (PMBP) with dibutyryl cyclic 
      AMP (dbcAMP) or forskolin and subsequently removed the dbcAMP or forskolin and 
      measured the proliferative response of the clones to antigen and 
      antigen-presenting cells (APC) in the presence or absence of exogenously added 
      interleukin-2 (IL-2). Cells precultured with dbcAMP or forskolin for 3 days did 
      not proliferate or produce IL-2 in response to antigen and APC, but did 
      proliferate to antigen and APC in the presence of IL-2. Cells that had not been 
      stimulated recently with antigen/APC or IL-2 were not affected by dbcAMP, while 
      cells stimulated recently with antigen/APC and IL-2 were susceptible to the 
      anergizing effect of dbcAMP. These observations support the hypothesis that 
      elevation in intracellular cAMP in antigen-activated Th1 clones, prior to 
      subsequent culture with antigen, induces a state of anergy.
FAU - Cone, R E
AU  - Cone RE
AD  - Department of Pathology, University of Connecticut Health Center, School of 
      Medicine and Dental Medicine, Farmington 06030-3105, USA.
FAU - Cochrane, R
AU  - Cochrane R
FAU - Lingenheld, E G
AU  - Lingenheld EG
FAU - Clark, R B
AU  - Clark RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Interleukin-2)
RN  - 0 (Myelin Basic Protein)
RN  - 1F7A44V6OU (Colforsin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Bucladesine/immunology
MH  - Clonal Anergy/*immunology
MH  - Clone Cells
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/agonists/*physiology
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Female
MH  - Interleukin-2/biosynthesis
MH  - Lymphocyte Activation
MH  - Mice
MH  - Myelin Basic Protein/immunology
MH  - Th1 Cells/drug effects/*immunology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S0008-8749(96)90274-9 [pii]
AID - 10.1006/cimm.1996.0274 [doi]
PST - ppublish
SO  - Cell Immunol. 1996 Nov 1;173(2):246-51. doi: 10.1006/cimm.1996.0274.