PMID- 8913724
OWN - NLM
STAT- MEDLINE
DCOM- 19970225
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 17
IP  - 2
DP  - 1996 Oct
TI  - Detection of chromosomal DNA gains and losses in testicular germ cell tumors by 
      comparative genomic hybridization.
PG  - 78-87
AB  - To extend the results of conventional cytogenetic analysis of testicular germ 
      cell tumors (TGCTs), we applied the new molecular cytogenetic method of 
      comparative genomic hybridization (CGH), which enables the detection of 
      chromosomal imbalances without the need for dividing cells. DNA from II TGCTs was 
      studied by CGH. In all tumors examined, gain of 12p, mostly of the whole p arm, 
      could be demonstrated. However, in three tumors, an amplification of 12p material 
      restricted to the chromosomal bands 12p11.2-p12.1 was found. Further fluorescence 
      in situ hybridization (FISH) analysis using a yeast artificial chromosome (YAC) 
      that was previously mapped to that region revealed multiple copies of that 
      chromosomal segment in interphase nuclei of these tumors. This finding is an 
      important clue to the localization of candidate protooncogenes at 12p involved in 
      TGCTs. Gains of small chromosomal regions at 2p, 4q, 6p, and 19p were also 
      detected recurrently. Furthermore, gains of chromosomes 8, 14, 21, and X as well 
      as loss of chromosome 13 were frequent findings. In conclusion, CGH provides new 
      insights into genetic alterations of TGCTs. By using CGH, chromosomal subregions 
      could be identified that may harbor genes involved in the pathogenesis of this 
      malignancy.
FAU - Korn, W M
AU  - Korn WM
AD  - West German Cancer Center, University of Essen, Medical School, Federal Republic 
      of Germany.
FAU - Oide Weghuis, D E
AU  - Oide Weghuis DE
FAU - Suijkerbuijk, R F
AU  - Suijkerbuijk RF
FAU - Schmidt, U
AU  - Schmidt U
FAU - Otto, T
AU  - Otto T
FAU - du Manoir, S
AU  - du Manoir S
FAU - Geurts van Kessel, A
AU  - Geurts van Kessel A
FAU - Harstrick, A
AU  - Harstrick A
FAU - Seeber, S
AU  - Seeber S
FAU - Becher, R
AU  - Becher R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Chromosome Aberrations/genetics
MH  - *Chromosomes, Human, Pair 12/genetics
MH  - Chromosomes, Human, Pair 4
MH  - Chromosomes, Human, Pair 6
MH  - DNA, Neoplasm/*analysis
MH  - Gene Amplification
MH  - Germinoma/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Testicular Neoplasms/*genetics
EDAT- 1996/10/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199610)17:2<78::AID-GCC2>3.0.CO;2-Y [pii]
AID - 10.1002/(SICI)1098-2264(199610)17:2<78::AID-GCC2>3.0.CO;2-Y [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Oct;17(2):78-87. doi: 
      10.1002/(SICI)1098-2264(199610)17:2<78::AID-GCC2>3.0.CO;2-Y.