PMID- 8914510
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20240109
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 252
IP  - 5
DP  - 1996 Oct 16
TI  - Interphase fluorescence in situ hybridization mapping: a physical mapping 
      strategy for plant species with large complex genomes.
PG  - 497-502
AB  - The chromatin in interphase nuclei is much less condensed than are metaphase 
      chromosomes, making the resolving power of fluorescence in situ hybridization 
      (FISH) two orders of magnitude higher in interphase nuclei than on metaphase 
      chromosomes. In mammalian species it has been demonstrated that within a certain 
      range the interphase distance between two FISH sites can be used to estimate the 
      linear DNA distance between the two probes. The interphase mapping strategy has 
      never been applied in plant species, mainly because of the low sensitivity of the 
      FISH technique on plant chromosomes. Using a CCD (charge-coupled device) camera 
      system, we demonstrate that DNA probes in the 4 to 8 kb range can be detected on 
      both metaphase and interphase chromosomes in maize. DNA probes pA1-Lc and 
      pSh2.5.SstISalI, which contain the maize loci a1 and sh2, respectively, and are 
      separated by 140 kb, completely overlapped on metaphase chromosomes. However, 
      when the two probes were mapped in interphase nuclei, the FISH signals were well 
      separated from each other in 86% of the FISH sites analyzed. The average 
      interphase distance between the two probes was 0.50 micron. This result suggests 
      that the resolving power of interphase FISH mapping in plant species can be as 
      little as 100 kb. We also mapped the interphase locations of another pair of 
      probes, ksu3/4 and ksu16, which span the Rp1 complex controlling rust resistance 
      of maize. Probes ksu3/4 and ksu16 were mapped genetically approximately 4 cM 
      apart and their FISH signals were also overlapped on metaphase chromosomes. These 
      two probes were separated by an average of 2.32 microns in interphase nuclei. The 
      possibility of estimating the linear DNA distance between ksu3/4 and ksu16 is 
      discussed.
FAU - Jiang, J
AU  - Jiang J
AD  - Department of Horticulture, University of Wisconsin-Madison 53706, USA.
FAU - Hulbert, S H
AU  - Hulbert SH
FAU - Gill, B S
AU  - Gill BS
FAU - Ward, D C
AU  - Ward DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Cell Nucleus/genetics
MH  - Chromosome Mapping/instrumentation/*methods
MH  - DNA Probes
MH  - DNA, Plant/*genetics
MH  - Genes, Plant
MH  - Genetic Markers
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Metaphase
MH  - Multigene Family
MH  - Zea mays/genetics
EDAT- 1996/10/16 00:00
MHDA- 1996/10/16 00:01
CRDT- 1996/10/16 00:00
PHST- 1996/10/16 00:00 [pubmed]
PHST- 1996/10/16 00:01 [medline]
PHST- 1996/10/16 00:00 [entrez]
AID - 10.1007/BF02172395 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1996 Oct 16;252(5):497-502. doi: 10.1007/BF02172395.