PMID- 8914827
OWN - NLM
STAT- MEDLINE
DCOM- 19970505
LR  - 20061115
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 25
IP  - 3
DP  - 1996 Nov 1
TI  - Interactive computer-aided assignment of multiple probes to cytogenetic bands by 
      simultaneous dual color fluorescence in situ hybridization and DAPI banding.
PG  - 295-300
AB  - A macro function was developed to run in conjunction with the popular image 
      analysis package NIH Image, to allow simultaneous determination of mapping 
      positions of one or two separate probes with respect to cytogenetic bands by dual 
      color fluorescence in situ hybridization (FISH) and DAPI banding, and by 
      determination of their fractional distance from pter (FLpter). In order to allow 
      maximal flexibility, a user-defined line along the chromosome is used for 
      measurements. Algorithms were developed to detect the ends of the chromosome and 
      the cytogenetic bands. Results of the analysis are presented in graphical form, 
      comprising a display of the DAPI intensity along the chromosome, the positions of 
      the probe(s), the locations of bands as determined by analysis of the second 
      derivative of the DAPI intensity profile, and a standard ideogram of the 
      chromosome for comparison. The approach was validated and compared to visual 
      assignment of probes to DAPI bands using the cosmid clone PYGM, which has been 
      previously mapped to chromosome 11q13, and has been used as a landmark for 
      mapping for other probes.
FAU - Burde, S
AU  - Burde S
AD  - Los Alamos National Laboratory, Life Sciences Division, Cytometry Group, New 
      Mexico 87545, USA. burde@lanl.gov
FAU - Joss, G
AU  - Joss G
FAU - Gonzales, J A
AU  - Gonzales JA
FAU - Coulon, C H
AU  - Coulon CH
FAU - Park, M S
AU  - Park MS
FAU - Deaven, L L
AU  - Deaven LL
FAU - Marrone, B L
AU  - Marrone BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Cells, Cultured
MH  - *Chromosome Banding
MH  - Cosmids
MH  - Fibroblasts/cytology
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Indoles/*chemistry
MH  - Skin
EDAT- 1996/11/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(19961101)25:3<295::AID-CYTO11>3.0.CO;2-R [pii]
AID - 10.1002/(SICI)1097-0320(19961101)25:3<295::AID-CYTO11>3.0.CO;2-R [doi]
PST - ppublish
SO  - Cytometry. 1996 Nov 1;25(3):295-300. doi: 
      10.1002/(SICI)1097-0320(19961101)25:3<295::AID-CYTO11>3.0.CO;2-R.