PMID- 8915312
OWN - NLM
STAT- MEDLINE
DCOM- 19961224
LR  - 20080317
IS  - 0003-987X (Print)
IS  - 0003-987X (Linking)
VI  - 132
IP  - 11
DP  - 1996 Nov
TI  - Detection of human T-cell lymphotrophic virus type I in archival tissue 
      specimens.
PG  - 1339-43
AB  - OBJECTIVE AND DESIGN: To develop a method for the detection of human T-cell 
      lymphotropic virus type I (HTLV-I) in archival biopsy specimens. A polymerase 
      chain reaction-based gene amplification method was developed to detect HTLV-I 
      proviral DNA in paraffin-embedded specimens. The specificity of the polymerase 
      chain reaction products was controlled by Southern blot analysis using a nested 
      oligonucleotide probe and by nucleotide sequencing. The nucleophosmin gene and 
      the T-cell receptor-gamma gene were used as controls for the integrity and 
      adequacy of total DNA and T-cell DNA, respectively. This study was conducted with 
      patients referred to an academic medical center. Biopsy specimens were obtained 
      from lesional skin or lymph node from Japanese patients with HTLV-I seropositive 
      adult T-cell leukemia/lymphoma. The main outcome measure was the ability to 
      detect HTLV-I pX region proviral DNA. RESULTS: Comparative analysis of DNA 
      extracted from fresh samples of the HTLV-I infected MT4T-cell line demonstrated 
      that formalin fixation and paraffin embedding resulted in a 100-fold reduction in 
      sensitivity of the assay. Nevertheless, HTLV-I pX sequences were still readily 
      detectable in paraffin-embedded samples of MT4 T cells and adult T-cell 
      leukemia/lymphoma specimens. Both formalin and B5 fixation were suitable for the 
      assay that was 100% specific for HTLV-I-infected tissues. CONCLUSIONS: The use of 
      this method should greatly facilitate investigation of the role of HTLV-I in 
      human diseases by allowing analysis of a wide variety of archival tissue 
      specimens. In addition, the controls designed for the current study can be used 
      in a variety of other polymerase chain reaction-based studies of T cells to 
      ensure against false-negative results caused by DNA degradation or inadequate 
      T-cell density.
FAU - Wood, G S
AU  - Wood GS
AD  - Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
FAU - Ruffo, A
AU  - Ruffo A
FAU - Salvekar, A
AU  - Salvekar A
FAU - Henghold, W
AU  - Henghold W
FAU - Takeshita, M
AU  - Takeshita M
FAU - Kikuchi, M
AU  - Kikuchi M
LA  - eng
GR  - AR39750/AR/NIAMS NIH HHS/United States
GR  - AR40844/AR/NIAMS NIH HHS/United States
GR  - CA60014/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Dermatol
JT  - Archives of dermatology
JID - 0372433
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - DNA Probes
MH  - DNA, Viral/analysis
MH  - Human T-lymphotropic virus 1/genetics/*isolation & purification
MH  - Humans
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - Arch Dermatol. 1996 Nov;132(11):1339-43.