PMID- 8916963
OWN - NLM
STAT- MEDLINE
DCOM- 19961217
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 10
DP  - 1996 Nov 15
TI  - Design and validation of DNA probe sets for a comprehensive interphase 
      cytogenetic analysis of acute myeloid leukemia.
PG  - 3962-71
AB  - The objective of this study was to design DNA probe sets that enable the 
      detection of chromosome aberrations in acute myeloid leukemia (AML) by interphase 
      cytogenetics using fluorescence in situ hybridization (FISH) and to compare the 
      results of interphase cytogenetics with those of conventional chromosome banding 
      analysis. One hundred five consecutive patients with adult AML entered on a 
      multicenter treatment trial were studied with a comprehensive set of DNA probes 
      recognizing the most relevant AML-associated structural and numerical chromosome 
      aberrations: translocations t(8;21), t(15;17), and t(11q23); inversion 
      inv(16);chromosomal deletions (5q-, 7q-, 9q-, 12p-, 13q-, 17p-, and 20q-); and 
      chromosomal aneuploidies. Interphase cytogenetics was particularly sensitive for 
      detecting the AML-specific gene fusions: 3 additional cases of inv(16) and 1 
      additional case of t(8;21) were identified by FISH that were missed by banding 
      analysis, whereas equal numbers of t(11q23) and t(15;17) were detected. Five 
      additional cases of trisomy 8q, 3 more cases of trisomy 11q, and 2 more cases of 
      trisomies 21q and 22q were shown by FISH. These aberrations were either masked in 
      complex karyo-types or identified in cases in which conventional banding analysis 
      failed. On the other hand, the DNA probes selected were not informative to detect 
      1 case of 5q-, 9q-, and 20q-. In 5 cases, clonal aberrations were detected on 
      banding analysis for which no FISH probes were selected. In conclusion, 
      interphase cytogenetics proved to be more sensitive for detecting AML-specific 
      chimeric gene fusions and some partial trisomies. Interphase cytogenetics 
      provides a powerful technique complementary and, with further development of 
      diagnostic DNA probes, even an alternative to chromosome banding studies for the 
      cytogenetic analysis of AML.
FAU - Fischer, K
AU  - Fischer K
AD  - Medizinische Klinik and Poliklinik V, University of Heidelberg, Germany.
FAU - Scholl, C
AU  - Scholl C
FAU - Salat, J
AU  - Salat J
FAU - Frohling, S
AU  - Frohling S
FAU - Schlenk, R
AU  - Schlenk R
FAU - Bentz, M
AU  - Bentz M
FAU - Stilgenbauer, S
AU  - Stilgenbauer S
FAU - Lichter, P
AU  - Lichter P
FAU - Dohner, H
AU  - Dohner H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Acute Disease
MH  - *Chromosome Aberrations
MH  - *DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics/pathology
MH  - Male
MH  - Metaphase
MH  - Oncogenes
EDAT- 1996/11/15 00:00
MHDA- 1996/11/15 00:01
CRDT- 1996/11/15 00:00
PHST- 1996/11/15 00:00 [pubmed]
PHST- 1996/11/15 00:01 [medline]
PHST- 1996/11/15 00:00 [entrez]
AID - S0006-4971(20)61321-0 [pii]
PST - ppublish
SO  - Blood. 1996 Nov 15;88(10):3962-71.