PMID- 8916970
OWN - NLM
STAT- MEDLINE
DCOM- 19961217
LR  - 20220410
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 10
DP  - 1996 Nov 15
TI  - Generation of CD1+RelB+ dendritic cells and tartrate-resistant acid 
      phosphatase-positive osteoclast-like multinucleated giant cells from human 
      monocytes.
PG  - 4029-39
AB  - We previously showed that granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) and macrophage colony-stimulating factor (M-CSF) stimulate the 
      differentiation of human monocytes into two phenotypically distinct types of 
      macrophages. However, in vivo, not only CSF but also many other cytokines are 
      produced under various conditions. Those cytokines may modulate the 
      differentiation of monocytes by CSFs. In the present study, we showed that CD14+ 
      adherent human monocytes can differentiate into CD1+relB+ dendritic cells (DC) by 
      the combination of GM-CSF plus interleukin-4 (IL-4) and that they differentiate 
      into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like 
      multinucleated giant cells (MGC) by the combination of M-CSF plus IL-4. However, 
      the monocyte-derived DC were not terminally differentiated cells; they could 
      still convert to macrophages in response to M-CSF. Tumor necrosis factor-alpha 
      (TNF-alpha) stimulated the terminal differentiation of the DC by downregulating 
      the expression of the M-CSF receptor, cfms mRNA, and aborting the potential to 
      convert to macrophages. In contrast to IL-4, interferon-gamma (IFN-gamma) had no 
      demonstrable effect on the differentiation of monocytes. Rather, IFN-gamma 
      antagonized the effect of IL-4 and suppressed the DC and MGC formation induced by 
      GM-CSF + IL-4 and M-CSF + IL-4, respectively. Taken together, these results 
      provide a new aspect to our knowledge of monocyte differentiation and provide 
      evidence that human monocytes are flexible in their differentiation potential and 
      are precursors not only of macrophages but also of CD1+relB+DC and TRAP-positive 
      MGC. Such a diverse pathway of monocyte differentiation may constitute one of the 
      basic mechanisms of immune regulation.
FAU - Akagawa, K S
AU  - Akagawa KS
AD  - Department of Immunology, National Institute of Health, Tokyo, Japan.
FAU - Takasuka, N
AU  - Takasuka N
FAU - Nozaki, Y
AU  - Nozaki Y
FAU - Komuro, I
AU  - Komuro I
FAU - Azuma, M
AU  - Azuma M
FAU - Ueda, M
AU  - Ueda M
FAU - Naito, M
AU  - Naito M
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD1)
RN  - 0 (Biomarkers)
RN  - 0 (Isoenzymes)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RELB protein, human)
RN  - 0 (Transcription Factors)
RN  - 147337-75-5 (Transcription Factor RelB)
RN  - 207137-56-2 (Interleukin-4)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/*analysis
MH  - Antigens, CD1/*analysis
MH  - Biomarkers
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/chemistry/*cytology
MH  - Giant Cells/*cytology/enzymology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-4/*pharmacology
MH  - Isoenzymes/*analysis
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Macrophages/classification/cytology
MH  - Monocytes/cytology/*drug effects
MH  - Osteoclasts/*cytology/enzymology
MH  - *Proto-Oncogene Proteins
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Transcription Factor RelB
MH  - Transcription Factors/*analysis
EDAT- 1996/11/15 00:00
MHDA- 1996/11/15 00:01
CRDT- 1996/11/15 00:00
PHST- 1996/11/15 00:00 [pubmed]
PHST- 1996/11/15 00:01 [medline]
PHST- 1996/11/15 00:00 [entrez]
AID - S0006-4971(20)61328-3 [pii]
PST - ppublish
SO  - Blood. 1996 Nov 15;88(10):4029-39.