PMID- 8917347
OWN - NLM
STAT- MEDLINE
DCOM- 19961218
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 16
IP  - 5A
DP  - 1996 Sep-Oct
TI  - A rapid and simplified technique for analysis of archival formalin-fixed, 
      paraffin-embedded tissue by fluorescence in situ hybridization (FISH).
PG  - 2533-6
AB  - We here present a simplification of the entire procedure for preparing 
      formalin-fixed, paraffin-embedded tissue to be used for FISH-analysis. The steps 
      for deparaffinisation and disintegration of the tissue to produce intact cell 
      nuclei in a monodispersed suspension are detailed as well as the hybridisation 
      steps. The procedure results in a clear cell suspension with intact cell nuclei 
      and without clumped debris particles. The enzymatic digestion is restricted to 
      the trypsinisation step of the deparaffinisation procedure. No further 
      pretreatments prior to hybridization are performed. This modified technique has 
      been successfully applied to different formalin-fixed, paraffin-embedded 
      materials.
FAU - Kopf, I
AU  - Kopf I
AD  - Department of Oncology, Goteborg University, Sweden.
FAU - Hanson, C
AU  - Hanson C
FAU - Delle, U
AU  - Delle U
FAU - Verbiene, I
AU  - Verbiene I
FAU - Weimarck, A
AU  - Weimarck A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
SB  - IM
MH  - Cell Nucleus/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Paraffin Embedding
MH  - Specimen Handling/*methods
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1996 Sep-Oct;16(5A):2533-6.