PMID- 8918373
OWN - NLM
STAT- MEDLINE
DCOM- 19961218
LR  - 20071114
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 15
IP  - 5
DP  - 1996 Nov
TI  - Differential regulation of airway epithelial integrins by growth factors.
PG  - 664-72
AB  - The pattern of integrin expression on human airway epithelium changes 
      significantly in injury or inflammation. In particular, two integrins, the 
      fibronectin receptor, alpha 5 beta 1 and the fibronectin/tenascin receptor alpha 
      v beta 6, are expressed at low or undetectable levels in normal airways in vivo 
      but are induced in response to airway epithelial injury. We investigated the 
      effects of various growth factors known to be present in the airways on the 
      expression of constitutively expressed and inducible airway epithelial integrins 
      using flow cytometry. In primary cultures of human airway epithelial cells, 
      transforming growth factor-beta 1 (TGF beta 1) dramatically increased expression 
      of alpha v beta 6 and essentially did not affect the expression of any other 
      integrin, including alpha 5 beta 1. In contrast, epidermal growth factor (EGF) 
      upregulated surface levels of both alpha v beta 6 and alpha 5 beta 1. Together, 
      TGF beta 1 and EGF had an additive effect on alpha v beta 6 and alpha 5 beta 1 
      expression while increasing levels of alpha 2 beta 1 and decreasing expression of 
      alpha 3 beta 1- and alpha 6-containing integrins. In contrast, the transformed 
      airway epithelial cell line, BEAS-2B, expressed a markedly different repertoire 
      of integrins. Integrin expression on BEAS-2B cells was not affected by any of the 
      growth factors tested in this study. These results demonstrate that, in primary 
      cultures of human airway epithelial cells, the pattern of integrin expression can 
      be dramatically altered by growth factors. The inducible integrins, alpha v beta 
      6, and alpha 5 beta 1 are most subject to regulation by growth factors and 
      expression of each of these can be differentially regulated. The differential 
      regulation of the two principal fibronectin receptors on airway epithelial cells 
      suggests that they may mediate different cellular responses to fibronectin.
FAU - Wang, A
AU  - Wang A
AD  - Department of Medicine, San Francisco General Hospital, California, USA.
FAU - Yokosaki, Y
AU  - Yokosaki Y
FAU - Ferrando, R
AU  - Ferrando R
FAU - Balmes, J
AU  - Balmes J
FAU - Sheppard, D
AU  - Sheppard D
LA  - eng
GR  - HL/AI33259/HL/NHLBI NIH HHS/United States
GR  - HL02765-02/HL/NHLBI NIH HHS/United States
GR  - HL47412/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Growth Substances)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Integrins)
RN  - 0 (Receptors, Fibronectin)
RN  - 0 (integrin alpha9)
RN  - 0 (integrin alphavbeta6)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - *Antigens, Neoplasm
MH  - Bronchi/*chemistry/cytology/drug effects
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Epithelial Cells
MH  - Epithelium/chemistry/drug effects
MH  - Growth Substances/*pharmacology
MH  - Humans
MH  - *Integrin alpha Chains
MH  - Integrins/*analysis
MH  - Receptors, Fibronectin/*analysis
MH  - Up-Regulation/drug effects
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1165/ajrcmb.15.5.8918373 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1996 Nov;15(5):664-72. doi: 
      10.1165/ajrcmb.15.5.8918373.