PMID- 8918585
OWN - NLM
STAT- MEDLINE
DCOM- 19961212
LR  - 20190512
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 106
IP  - 2
DP  - 1996 Nov
TI  - Structural peculiarities of a truncated V kappa III immunoglobulin light chain in 
      myeloma with light chain deposition disease.
PG  - 357-61
AB  - We report on the primary sequence of the monoclonal immunoglobulin light chain 
      (LC) REV involved in myeloma-associated light chain deposition disease (LCDD). 
      This sequence was deduced from that of the corresponding complementary (c)DNA in 
      bone marrow plasma cells. Products of three independent amplifications by 
      polymerase chain reaction (PCR) were sequenced and found to be identical. The 
      kappa mRNA encoding this N-glycosylated LC showed an overall normal structure 
      consisting of a V kappa III segment rearranged to J kappa II. Direct N-terminal 
      amino acid sequencing of the circulating monoclonal IgA2, kappa showed identity 
      with the bone marrow-derived sequence. The kappa-chain presented several unusual 
      features affecting both the leader sequence and the variable (V) region. Four 
      unique amino acid substitutions were found at positions -8, -3, -2 and -1 in the 
      leader sequence and probably resulted in an unusual cleavage by signal peptidase, 
      thus making the LC truncated by one residue and accounting for its unique 
      hydrophobic N-terminus: Ile-Ile-Leu. Additional peculiarities were observed in 
      the V region, including a Thr74-->Asn substitution creating a N-glycosylation 
      site, and Thr53-->Ile, which was only reported once among human kappa III chains, 
      in another LCDD case, and may be of special significance at a position usually 
      harbouring a polar amino acid.
FAU - Decourt, C
AU  - Decourt C
AD  - Laboratoire d'Immunologie et d'Immunogenetique, CNRS EP J0118, Faculte de 
      Medecine, Limoges, France.
FAU - Cogne, M
AU  - Cogne M
FAU - Rocca, A
AU  - Rocca A
LA  - eng
SI  - GENBANK/S83559
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (DNA Primers)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Immunoglobulin kappa-Chains)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Bone Marrow
MH  - DNA Primers
MH  - Gene Rearrangement/genetics
MH  - Genes, Immunoglobulin/genetics
MH  - Humans
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin Variable Region/genetics
MH  - Immunoglobulin kappa-Chains/*genetics/immunology
MH  - Molecular Sequence Data
MH  - Multiple Myeloma/*genetics/immunology
MH  - Paraproteinemias/*genetics/immunology
MH  - Polymerase Chain Reaction
MH  - Protein Sorting Signals/genetics
MH  - RNA, Messenger/analysis
MH  - Sequence Homology, Amino Acid
PMC - PMC2200580
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
PMCR- 1997/11/01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PHST- 1997/11/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1996.d01-841.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1996 Nov;106(2):357-61. doi: 
      10.1046/j.1365-2249.1996.d01-841.x.