PMID- 8918905
OWN - NLM
STAT- MEDLINE
DCOM- 19961231
LR  - 20190606
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 44
IP  - 11
DP  - 1996 Nov
TI  - Classical morphology, esterase cytochemistry, and interphase cytogenetics of 
      peripheral blood and bone marrow smears.
PG  - 1303-9
AB  - We used peripheral blood (PB) and bone marrow (BM) smears in the development of 
      two methods based on cytomorphology and esterase cytochemistry in combination 
      with fluorescence in situ hybridization (FISH). The first method involves 
      photodocumentation of May-Grunewald-Giemsa (MGG)-stained cells, followed by 
      destaining in methanol-acetic acid, fixation in paraformaldehyde, and digestion 
      with protease and RNAse before FISH using alpha-satellite probes that specify 
      chromosomes X, 7, 8, and 17. On average, two hybridization signals were seen in 
      94.5% of disomic BM cells. The hybridization sensitivity was found to vary, 
      however, both among morphologically defined hematopoietic cell lineages and among 
      differentation levels within a lineage. In the second method, an esterase 
      staining technique was followed by the same treatment as for MGG-stained cells. 
      The esterases and FISH signals could be simultaneously visualized and the method 
      was found suitable for rapid screening of in situ signals in cytochemically 
      defined granulocytes and lymphocytes but not in monocytes. The combined methods 
      proved very useful in elucidating the clinical significance of chromosomal 
      abnormalities seen in two cases of leukemia.
FAU - Jacobsson, B
AU  - Jacobsson B
AD  - Department of Clinical Pathology, Karolinska Institute at Danderyd Hospital, 
      Sweden.
FAU - Bernell, P
AU  - Bernell P
FAU - Arvidsson, I
AU  - Arvidsson I
FAU - Hast, R
AU  - Hast R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - EC 3.1.- (Esterases)
SB  - IM
MH  - Blood Cells/enzymology/*pathology
MH  - Bone Marrow/enzymology/*pathology
MH  - Cell Cycle/genetics
MH  - *Chromosomes, Human
MH  - Esterases/*analysis
MH  - Histocytochemistry/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Leukemia, Myeloid, Acute/enzymology/genetics/pathology
MH  - Leukemia, Myelomonocytic, Chronic/enzymology/genetics/pathology
MH  - Staining and Labeling
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1177/44.11.8918905 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1996 Nov;44(11):1303-9. doi: 10.1177/44.11.8918905.