PMID- 8920998
OWN - NLM
STAT- MEDLINE
DCOM- 19961230
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 319 ( Pt 3)
IP  - Pt 3
DP  - 1996 Nov 1
TI  - The lysosomal Ca2+ pool in MDCK cells can be released by ins(1,4,5)P3-dependent 
      hormones or thapsigargin but does not activate store-operated Ca2+ entry.
PG  - 909-12
AB  - In several cell types, Ca2+ release from intracellular Ca2+ stores by 
      Ins(1,4,5)P3 elicits Ca2+ influx from the extracellular space into the cytoplasm, 
      termed store-operated Ca2+ entry (SOCE). In MDCK cells, the 
      Ins(1,4,5)P3-sensitive Ca2+ store giving rise to SOCE essentially overlaps with 
      the thapsigargin (TG)-sensitive store. Recent evidence suggests that in MDCK 
      cells lysosomes form a Ca2+ pool that is functionally coupled with the 
      Ins(1,4,5)P3-sensitive Ca2+ store: Ca2+ can be selectively released from 
      lysosomes by glycyl-L-phenylalanine naphthylamide, an agent inducing lysosomal 
      swelling with subsequent and reversible permeabilization of the vesicular 
      membranes. This compartment is also depleted by Ins(1,4,5)P3-dependent agonists 
      or TG, indicating that it is part of a larger, Ins(1,4,5)P3-sensitive Ca2+ pool. 
      Here we show that whereas SOCE is triggered by Ca2+ release from the entire 
      Ins(1,4,5)P3-sensitive Ca2+ pool, selective Ca2+ release from lysosomes alone is 
      unable to trigger SOCE. This finding is consistent with measurements of the 
      store-operated cation current, a direct parameter for store-operated Ca2+ and Na+ 
      entry into MDCK cells. Hence it is proposed that the Ins(1,4,5)P3-sensitive Ca2+ 
      pool is composed of different intracellular compartments that do not uniformly 
      stimulate Ca2+ entry into the cell.
FAU - Haller, T
AU  - Haller T
AD  - Department of Physiology, University of Innsbruck, Austria.
FAU - Volkl, H
AU  - Volkl H
FAU - Deetjen, P
AU  - Deetjen P
FAU - Dietl, P
AU  - Dietl P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Dipeptides)
RN  - 21438-66-4 (glycylphenylalanine 2-naphthylamide)
RN  - 67526-95-8 (Thapsigargin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - 9NEZ333N27 (Sodium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Dipeptides/pharmacology
MH  - Dogs
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Intracellular Membranes/drug effects/physiology
MH  - Kidney
MH  - Kinetics
MH  - Lysosomes/drug effects/*physiology
MH  - Membrane Potentials
MH  - Sodium/metabolism
MH  - Thapsigargin/*pharmacology
PMC - PMC1217874
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
PMCR- 1997/05/01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PHST- 1997/05/01 00:00 [pmc-release]
AID - 10.1042/bj3190909 [doi]
PST - ppublish
SO  - Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):909-12. doi: 10.1042/bj3190909.