PMID- 8921170
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20221207
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 241
IP  - 1
DP  - 1996 Oct 1
TI  - Cloning of murine LAG-3 by magnetic bead bound homologous probes and PCR 
      (gene-capture PCR).
PG  - 93-102
AB  - In recent years PCR-based gene cloning strategies have found wide application in 
      molecular biology, due to the power, speed, and relative simplicity of the PCR 
      methodology. We have set up a novel PCR cloning strategy to isolate homologous 
      genes, which is based on the capture of the cDNA sequence(s) of interest with a 
      biotinylated probe and streptavidin-coupled magnetic beads followed by PCR 
      amplification of the selected molecules. This method does not require sequence 
      information on 5' and 3' regions of the cDNA of interest and permits gene 
      isolation to be sensitive, fast, simple, and specific even when the conventional 
      screening procedures give rise to high backgrounds. By using this technique, 
      which we propose to call gene-capture PCR (GC-PCR) cloning, we were able to 
      isolate the full-length murine lymphocyte activation gene 3 (LAG-3) cDNA from 
      total RNA of activated thymocytes. The GC-PCR technique represents a powerful 
      tool for easy isolation not only of homologous genes from related species, but 
      also of genes sharing conserved regions of suitable length, gene variants, and 
      gene encoding proteins where only limited knowledge of the amino acid sequence 
      exists.
FAU - Mastrangeli, R
AU  - Mastrangeli R
AD  - Istituto di Ricerca Cesare Serono SpA, Ardea, Italy.
FAU - Micangeli, E
AU  - Micangeli E
FAU - Donini, S
AU  - Donini S
LA  - eng
SI  - GENBANK/X98113
PT  - Journal Article
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Antigens, CD)
RN  - 0 (Membrane Proteins)
RN  - 0 (Lymphocyte Activation Gene 3 Protein)
RN  - 0 (Lag3 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, CD
MH  - Base Sequence
MH  - Cloning, Molecular/*methods
MH  - Immunomagnetic Separation/methods
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Molecular Probe Techniques
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Lymphocyte Activation Gene 3 Protein
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0003-2697(96)90382-6 [pii]
AID - 10.1006/abio.1996.0382 [doi]
PST - ppublish
SO  - Anal Biochem. 1996 Oct 1;241(1):93-102. doi: 10.1006/abio.1996.0382.