PMID- 8924917
OWN - NLM
STAT- MEDLINE
DCOM- 19961028
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 19
IP  - 3
DP  - 1996 Mar
TI  - Stimulation of macrophage DNA synthesis by polyanionic substances through binding 
      to the macrophage scavenger receptor.
PG  - 449-55
AB  - We previously demonstrated that ligands of macrophage scavenger receptors such as 
      acetylated low density lipoprotein (LDL), oxidized LDL and advanced glycation-end 
      products (AGE) of the Maillard reaction induce the growth of peritoneal exudate 
      macrophages, and that the activity of AGE is inhibited by the presence of an 
      antibody for granulocyte/macrophage colony-stimulating factor (GM-CSF). To 
      evaluate the suggested role of the scavenger receptor in the induction of 
      macrophage growth, we compared the effect of various polyanionic compounds which 
      were reported to either have or not to have competent activity for the binding of 
      acetylated LDL to scavenger receptors on macrophage DNA synthesis. Among the 
      polyanions exhibiting such activity, polyguanilic acid (poly G) and dextran 
      sulfate strongly augmented macrophage DNA synthesis, although they did not 
      increase macrophage cell number. On the other hand, polyanions which are not 
      ligands for the scavenger receptors did not show a significant augmenting effect, 
      suggesting that the binding of polyanions to the scavenger receptor is important 
      but not, by itself, sufficient. The augmentation of DNA synthesis in macrophages 
      cultured with dextran sulfate or poly G was inhibited by the co-presence of 
      anti-GM-CSF antibody, suggesting that the reaction is mediated by GM-CSF. 
      However, dextran sulfate did not augment the production of GM-CSF in macrophages. 
      Therefore, GM-CSF spontaneously present in macrophages might be a prerequisite 
      for the induction of DNA synthesis.
FAU - Sasaki, T
AU  - Sasaki T
AD  - Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
FAU - Horiuchi, S
AU  - Horiuchi S
FAU - Yamazaki, M
AU  - Yamazaki M
FAU - Yui, S
AU  - Yui S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Scavenger)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9007-49-2 (DNA)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cell Count
MH  - Cells, Cultured
MH  - DNA/*biosynthesis
MH  - Dextran Sulfate/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis
MH  - Lipopolysaccharides/chemistry
MH  - Lipoproteins, LDL/metabolism
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Polymers/*pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Scavenger
MH  - Stimulation, Chemical
MH  - Thymidine/metabolism
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1248/bpb.19.449 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1996 Mar;19(3):449-55. doi: 10.1248/bpb.19.449.