PMID- 8927695
OWN - NLM
STAT- MEDLINE
DCOM- 19961114
LR  - 20061115
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 145
IP  - 3
DP  - 1996 Mar
TI  - Rejoining and misrejoining of radiation-induced chromatin breaks. II. Biophysical 
      Model.
PG  - 281-8
AB  - A biophysical model for the kinetics of the formation of radiation-induced 
      chromosome aberrations is developed to account for the recent experimental 
      results obtained with a combination of the premature chromosome condensation 
      (PCC) and fluorescence in situ hybridization (FISH) techniques. In this model, we 
      consider the broken ends of DNA double-strand breaks (DSBs) to be reactant and 
      make use of the interaction distance hypothesis. The repair/misrepair process 
      between broken ends is suggested to consist of two steps; the first step 
      represents the two break ends approaching each other, and the second step 
      represents the enzymatic processes leading to DNA end-to-end rejoining. Only the 
      second step is reflected in the kinetics observed in experiments using PCC. The 
      model appears to be able to fit existing data for human cells. It is shown that 
      the kinetics of the formation of chromosome aberrations can be explained by a 
      single rate that characterizes both rejoining and misrejoining of DSBs, 
      suggesting that repair and misrepair share the same mechanism. Fast repair 
      (completed in minutes) in a subset of DSBs is suggested as an explanation of the 
      complete exchanges observed with PCC in human lymphocytes immediately after 
      irradiation. The fast repair component seems to be absent in human fibroblasts.
FAU - Wu, H
AU  - Wu H
AD  - NASA Lyndon B. Johnson Space Center, Radiation Biophysics Laboratory, Houston, 
      Texas 77058, USA.
FAU - Durante, M
AU  - Durante M
FAU - George, K
AU  - George K
FAU - Goodwin, E H
AU  - Goodwin EH
FAU - Yang, T C
AU  - Yang TC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Line
MH  - Chromatin/physiology/*radiation effects
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 4/*radiation effects
MH  - DNA/radiation effects
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Fibroblasts/radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Lymphocytes/cytology/radiation effects
MH  - Mathematics
MH  - Models, Genetic
MH  - *Models, Theoretical
MH  - Time Factors
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1996 Mar;145(3):281-8.