PMID- 8930889
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20191101
IS  - 1073-9688 (Print)
IS  - 1073-9688 (Linking)
VI  - 3
IP  - 3
DP  - 1996 Sep
TI  - Neutrophil-endothelial interactions in a cell-column model of the 
      microvasculature: effects of fMLP.
PG  - 329-42
AB  - OBJECTIVE: The purpose of this study was to develop an in vitro model which, 
      compared to stagnant two-chamber systems, is more analogous to the rheological 
      conditions found in the vasculature and permits near simultaneous measurement of 
      polymorphonuclear leukocytes (PMN) entrapment and endothelial, monolayer 
      permeability. METHODS: A previously described cell-column model of the 
      vasculature was perfused with 2 x 10(6) PMN/ml at an average fluid velocity of 
      0.09 cm/s and an average estimated endothelial surface shear stress of 4.5 
      dyne/cm2 for a total of 60 min. PMN entrapment was estimated from counts of PMN 
      in this recirculating system. Bovine endothelial permeability was estimated from 
      an indicator dilution analysis of the three co-injected dyes: blue dextran 
      (molecular weight [MW]) 2 x 10(6), sodium fluorescein (MW 342) and cyanocobalamin 
      (MW 1355). RESULTS: Circulation of PMN through cell-columns did not activate PMN, 
      although the number of circulating PMN decreased at a rate of 14% per 65 cm2 of 
      endothelial surface in the first 15 min. Endothelial cell monolayer permeability 
      did not change within 60 min. Addition of formyl-methionyl-leucyl-phenylalanine 
      (fMLP) (10(-5) M) activated PMN and significantly increased entrapment of 
      circulating PMN to 27% per 65 cm2 in the first 15 min. Although the percentage of 
      circulating PMN entrapped increased, endothelial monolayer permeability was not 
      increased by either the addition of 10(-5)M fMLP or PMN + fMLP to the cell-column 
      perfusate. CONCLUSIONS: Over the 60-min period studied, entrapment of PMN within 
      a recirculating model of the microvasculature was not associated with an increase 
      in endothelial permeability even after PMN stimulation with fMLP.
FAU - Haselton, F R
AU  - Haselton FR
AD  - Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee 
      37235, USA.
FAU - Woodall, J H
AU  - Woodall JH
FAU - Alexander, J S
AU  - Alexander JS
LA  - eng
GR  - HL40554/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Microcirculation
JT  - Microcirculation (New York, N.Y. : 1994)
JID - 9434935
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*cytology/drug effects
MH  - Flow Cytometry
MH  - Leukocyte Elastase/metabolism
MH  - Microcirculation/drug effects
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*cytology/drug effects
MH  - Oxygen Consumption
MH  - Superoxides/metabolism
MH  - Viscosity
EDAT- 1996/09/01 00:00
MHDA- 2000/03/29 09:00
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 2000/03/29 09:00 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 10.3109/10739689609148306 [doi]
PST - ppublish
SO  - Microcirculation. 1996 Sep;3(3):329-42. doi: 10.3109/10739689609148306.