PMID- 8931294
OWN - NLM
STAT- MEDLINE
DCOM- 19970328
LR  - 20190914
IS  - 0300-5623 (Print)
IS  - 0300-5623 (Linking)
VI  - 24
IP  - 5
DP  - 1996
TI  - Cell proliferation in renal cell carcinoma--a comparative study of cell kinetic 
      methods.
PG  - 291-5
AB  - Four different methods of assessing cell proliferation in renal cell carcinoma 
      were compared in a total of 136 samples to analyze their degree of agreement and 
      usefulness. The methods compared were flow cytometric S-phase (S-FCM) analysis, 
      proliferating cell nuclear antigen expression detected by immunohistochemistry 
      (PCNA-IHC), in vivo iododeoxyuridine incorporation analyzed with 
      immunohistochemistry (IdUrd-IHC), and flow cytometry (IdUrd-FCM). The mean S-FCM 
      fraction was 5.9%, compared with a mean PCNA-IHC labeling index of 4.7%. The mean 
      labeling indices obtained by IdUrd-IHC and IdUrd-FCM were 1.2% and 1.7%, 
      respectively. The four methods correlated well with each other. When the methods 
      were compared according to Bland and Altman, good agreement was shown. A 
      statistically significant difference in proliferation between diploid and 
      aneuploid tumor samples was found with all methods (P < 0.001). The results 
      showed that the four different methods provided comparable information on 
      proliferative activity, although different cell cycle compartments were 
      monitored.
FAU - Larsson, P
AU  - Larsson P
AD  - Department of Urology and Andrology, Umea University, Sweden.
FAU - Roos, G
AU  - Roos G
FAU - Stenling, R
AU  - Stenling R
FAU - Wilson, G D
AU  - Wilson GD
FAU - Ljungberg, B
AU  - Ljungberg B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Urol Res
JT  - Urological research
JID - 0364311
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - LGP81V5245 (Idoxuridine)
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Renal Cell/immunology/metabolism/*pathology
MH  - *Cell Division
MH  - *Cytological Techniques
MH  - DNA, Neoplasm/analysis
MH  - Diploidy
MH  - Evaluation Studies as Topic
MH  - Flow Cytometry
MH  - Humans
MH  - Idoxuridine/metabolism
MH  - Immunohistochemistry
MH  - Kidney Neoplasms/immunology/metabolism/*pathology
MH  - Kinetics
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - S Phase
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF00304779 [doi]
PST - ppublish
SO  - Urol Res. 1996;24(5):291-5. doi: 10.1007/BF00304779.