PMID- 8931871
OWN - NLM
STAT- MEDLINE
DCOM- 19970328
LR  - 20190905
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 288
IP  - 11
DP  - 1996 Oct
TI  - Changes in keratinocyte differentiation following mild irritation by sodium 
      dodecyl sulphate.
PG  - 684-90
AB  - Although the induction of acute irritant dermatitis by detergents has been 
      studied extensively in recent years, our understanding of the cell biological 
      events in the repair phase, and its relevance for the development of chronic 
      irritant dermatitis is limited. Here we studied the reaction pattern of human 
      skin to short-term application of sodium dodecyl sulphate (SDS) in a model that 
      induced a minimal acute inflammatory reaction (absence of polymorphonuclear 
      leucocytes, PMN) and did not have cytopathic effects on the epidermal 
      keratinocytes as determined by histological investigation. All parameters were 
      measured up to 14 days after exposure to SDS. Application of SDS caused 
      disturbances of barrier function as measured by transepidermal water loss and had 
      vascular effects as judged by erythema. Several cell biological markers for 
      epidermal growth and differentiation were examined by immunohistochemistry. A 
      rapid and strong induction of the cornified envelope precursor protein involucrin 
      was seen in the stratum spinosum, with a peak at 24 h. Within 24 h a strong 
      upregulation of epidermal fatty acid binding protein (E-FABP) was noted, with a 
      peak at 7 days after injury. Cellular proliferation in the basal layer was 
      increased fivefold as assessed by nuclear staining for the Ki-67 antigen, showing 
      a peak at 48 h. Surprisingly, no significant induction of cytokeratin 16 and 
      SKALP/elafin expression, two markers associated with epidermal 
      hyper-proliferation and inflammation, was seen. These findings suggest that the 
      cellular changes following exposure to detergent are distinct from those seen in 
      other forms of skin injury. We would speculate that the epidermal response to 
      detergent exposure is primarily directed at restoration of barrier function.
FAU - Le, M
AU  - Le M
AD  - Department of Dermatology, University Hospital Nijmegen, The Netherlands.
FAU - Schalkwijk, J
AU  - Schalkwijk J
FAU - Siegenthaler, G
AU  - Siegenthaler G
FAU - van de Kerkhof, P C
AU  - van de Kerkhof PC
FAU - Veerkamp, J H
AU  - Veerkamp JH
FAU - van der Valk, P G
AU  - van der Valk PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Biomarkers)
RN  - 0 (Carrier Proteins)
RN  - 0 (Detergents)
RN  - 0 (FABP5 protein, human)
RN  - 0 (FABP7 protein, human)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
RN  - 0 (Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 60108-77-2 (involucrin)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Adult
MH  - Biomarkers
MH  - Carrier Proteins/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Dermatitis, Irritant/etiology/metabolism/pathology
MH  - Detergents/*toxicity
MH  - Epidermis/drug effects/metabolism/pathology
MH  - Erythema/etiology
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Female
MH  - Humans
MH  - Keratinocytes/*drug effects/metabolism/*pathology
MH  - Keratins/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Models, Biological
MH  - Myelin P2 Protein/metabolism
MH  - *Neoplasm Proteins
MH  - Protein Precursors/metabolism
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Proteins/metabolism
MH  - Sodium Dodecyl Sulfate/*toxicity
MH  - Time Factors
MH  - *Tumor Suppressor Proteins
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1007/BF02505278 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 1996 Oct;288(11):684-90. doi: 10.1007/BF02505278.