PMID- 8933654
OWN - NLM
STAT- MEDLINE
DCOM- 19970307
LR  - 20180216
IS  - 0025-7931 (Print)
IS  - 0025-7931 (Linking)
VI  - 63
IP  - 6
DP  - 1996
TI  - Eosinophil adhesion to cultured airway epithelial cells from guinea pig lungs.
PG  - 358-62
AB  - We examined the effects of histamine, tumor necrosis factor-alpha (TNF alpha), 
      interferon-gamma (IFN gamma), indomethacin and dexamethasone on the adherence of 
      eosinophils to airway epithelial cells. Epithelial cells were isolated from 
      guinea pig trachea through enzymatic digestion, and cultured until they showed 
      confluence. Eosinophils were taken by peritoneal lavage from guinea pigs 
      sensitized with horse serum and purified. The adhesion of eosinophils to cultured 
      epithelial cells was examined using 51Cr. The percentage adhesion was obtained by 
      dividing counts per minute of lysed eosinophils adhered to epithelial cells by 
      suspended eosinophils. Histamine significantly increased the adhesion of 
      eosinophils to epithelial cells, while TNF alpha or IFN gamma did not 
      significantly alter the adhesion. Indomethacin significantly inhibited the 
      adhesion of eosinophils to epithelial cells in both nontreated and 
      histamine-induced conditions. Dexamethasone also reduced adhesion, although it 
      did not reach statistical significance. These findings indicate that histamine 
      induced eosinophil adherence to airway epithelial cells, suggesting a role of 
      endogenous prostaglandins in adherence.
FAU - Masuda, T
AU  - Masuda T
AD  - First Department of Internal Medicine, Tohoku University School of Medicine, 
      Sendai, Japan.
FAU - Shimura, S
AU  - Shimura S
FAU - Maruyama, N
AU  - Maruyama N
FAU - Nara, M
AU  - Nara M
FAU - Shirato, K
AU  - Shirato K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Respiration
JT  - Respiration; international review of thoracic diseases
JID - 0137356
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 82115-62-6 (Interferon-gamma)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Eosinophils/*physiology
MH  - Epithelial Cells
MH  - Guinea Pigs
MH  - Indomethacin/pharmacology
MH  - Interferon-gamma/pharmacology
MH  - Lung/cytology
MH  - Trachea/*cytology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000196577 [doi]
PST - ppublish
SO  - Respiration. 1996;63(6):358-62. doi: 10.1159/000196577.