PMID- 8937889
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20190909
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 34
IP  - 1
DP  - 1996 Nov
TI  - Phenotypic analysis of lymphocyte subpopulations in lymph nodes draining the ear 
      following exposure to contact allergens and irritants.
PG  - 25-35
AB  - The murine local lymph node assay (LLNA) measures in vivo proliferation in 
      draining lymph nodes (DLN) following topical exposure to chemicals to assess 
      contact sensitization potential. However, proliferation has also been observed 
      with some irritants. To further characterize events in the DLN during the LLNA 
      and distinguish allergens from irritants, phenotypic analysis of lymphocyte 
      subsets was made following topical exposure. In preliminary studies, mice were 
      treated on the ears for 3 consecutive days, and 48 hr following the final 
      application, analysis of CD3, CD4, CD8, and B220 expression was evaluated by flow 
      cytometry. The allergens oxazolone (OXAZ) and picryl chloride (TNCB) and the 
      irritant benzalkonium chloride (BC) increased cell number compared to vehicle. 
      The increase in lymph node cellularity for these materials was due to an increase 
      in the total number of T and B lymphocytes. Interestingly, even though contact 
      sensitization is a cell-mediated immune response (Th1), mice exposed to the 
      contact allergens showed a preferential increase in B lymphocytes in the DLN as 
      seen by an increase in the percentage of B220+ cells. The percentage of B220+ 
      cells was 13.1 and 36.1% for OXA and TNCB, respectively, compared to percentages 
      of 7.4 and 9.3% for irritant and vehicle, respectively. With some allergens, a 
      concomitant decrease in the percentage of CD3+ cells was seen. Time course 
      studies demonstrated the increase in the percentage of B220+ cells was seen in 
      allergen treated mice by 24 hr after the final application of material, plateaued 
      by 48 hr, and was still elevated by 96 hr. In allergen-treated mice, percentages 
      of B220+ cells increased dose dependently. Further studies were performed to 
      evaluate additional contact allergens and irritants and determine if evaluation 
      of flow cytometric parameters could potentially identify contact allergens and 
      differentiate them from irritants. Analysis of data from these studies, which 
      examined a total of five contact allergens and six irritants, showed that the 
      modifications to the LLNA improved the identification of irritants and allergens 
      in individual experiments by including both phenotypic analysis of the DLN and 
      cell number per node as endpoints rather than either endpoint alone.
FAU - Sikorski, E E
AU  - Sikorski EE
AD  - Human Safety Department, Procter & Gamble Company, Cincinnati, Ohio, USA.
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Ryan, C A
AU  - Ryan CA
FAU - Miller, C M
AU  - Miller CM
FAU - Ridder, G M
AU  - Ridder GM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Allergens)
RN  - 0 (Irritants)
SB  - IM
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - *Ear
MH  - Female
MH  - Irritants/*administration & dosage
MH  - Lymph Nodes/*cytology
MH  - Lymphocyte Subsets/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred CBA
MH  - Phenotype
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S0272059096901724 [pii]
AID - 10.1006/faat.1996.0172 [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1996 Nov;34(1):25-35. doi: 10.1006/faat.1996.0172.