PMID- 8937892
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20190909
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 34
IP  - 1
DP  - 1996 Nov
TI  - Nitrofurazone: reproductive assessment by continuous breeding in Swiss mice.
PG  - 56-66
AB  - Nitrofurazone (NTFZ), a nitrofuran antibiotic, was evaluated for reproductive 
      toxicity in Swiss CD-1 mice using the Reproductive Assessment by Continuous 
      Breeding protocol. Male and female mice were cohabited for 15 weeks and exposed 
      to NTFZ in feed at concentrations of 0, 100, 375, and 750 ppm (14-102 mg/kg/day). 
      Fzero 750-ppm breeding pairs had significantly reduced fertility after 7 days of 
      exposure to NTFZ (17% fertile compared to 98% for control pairs) and were 
      infertile after the second litter. Fzero mid-dose pairs had progressively 
      decreasing fertility (47% by the fifth litter), reduced litter size, and reduced 
      proportion of pups born alive. Crossover breeding of control and high-dose Fzero 
      animals confirmed infertility in high-dose males and reduced litter size and pup 
      weight in high-dose females when compared to the control x control group. At 
      necropsy, there were no effects on body weight, but Fzero males had reduced 
      testis weight at the high dose and reduced epididymal sperm concentration and 
      abnormal sperm morphology at all doses of NTFZ. Increased liver as well as kidney 
      and adrenal weights (combined) were observed at 375 and 750 ppm; hepatic 
      hypertrophy was noted microscopically at 750 ppm. Fzero females had reduced body 
      weight, hepatic hypertrophy, and altered estrous cycles at 750 ppm and reduced 
      ovarian weight at all doses. In the second generation, F1 mice at 375 ppm had 
      reduced postnatal survival and body weight and produced smaller F2 litters 
      compared to control mice. At necropsy, F1 males had reduced testes weight and 
      epididymal sperm concentration, abnormal sperm morphology, hepatic hypertrophy at 
      375 ppm, and borderline nephropathy at 100 and 375 ppm. F1 females had decreased 
      body, liver, and ovarian weight at 375 ppm and altered estrous cycles at 100 and 
      375 ppm. Thus, NTFZ at > or = 100 ppm (> or = 14 mg/kg/day) caused adverse 
      reproductive effects in Fzero male and female and F1 female mice in the presence 
      of relatively mild systemic toxicity.
FAU - George, J D
AU  - George JD
AD  - Laboratory of Reproductive Toxicology, Research Triangle Institute, Research 
      Triangle Park, North Carolina 27709-2194, USA.
FAU - Fail, P A
AU  - Fail PA
FAU - Grizzle, T B
AU  - Grizzle TB
FAU - Heindel, J J
AU  - Heindel JJ
LA  - eng
GR  - N01-ES-6-5141/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Anti-Infective Agents)
RN  - X8XI70B5Z6 (Nitrofurazone)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/administration & dosage/*toxicity
MH  - Body Weight/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fertility/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nitrofurazone/administration & dosage/*toxicity
MH  - Organ Size/drug effects
MH  - Pregnancy
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S027205909690175X [pii]
AID - 10.1006/faat.1996.0175 [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1996 Nov;34(1):56-66. doi: 10.1006/faat.1996.0175.