PMID- 8939360
OWN - NLM
STAT- MEDLINE
DCOM- 19970227
LR  - 20200304
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 4
IP  - 7
DP  - 1996 Nov
TI  - Physical mapping of repetitive DNA sequences and 5S and 18S-26S rDNA in five wild 
      species of the genus Hordeum.
PG  - 491-9
AB  - The genetic relationships between several wild species and subspecies of the 
      genus Hordeum were assessed using fluorescence in situ hybridization (FISH). 
      Plant material included natural populations of wild barley growing in Spain of 
      the annual species, H. marinum ssp. marinum (2n = 14) and gussoneanum (2n = 14), 
      and H. murinum ssp. murinum (2n = 28), and leporinum (2n = 28) and the perennial 
      species H. bulbosum (2n = 14) and H. secalinum (2n = 28), plus the South American 
      perennial species H. chilense (2n = 14). FISH was used to locate the chromosomal 
      sites of two rDNA multigene families 5S and 18S-26S (pTa71 and pTa794) and three 
      repetitive DNA sequences (pSc119.2, pAs1 and pHch950) isolated from different 
      species and genera. The seven chromosomes of the diploid species were readily 
      distinguished by their external morphology and hybridization patterns to pTa71, 
      pTa794, pSc119.2 and pAs1. These DNA probes were also useful for the 
      identification of homologous chromosomes and in differentiating these from 
      unidentified chromosomes in the tetraploid taxa. The use of the probe pHch950 
      permitted intergenomic differentiation in tetraploids and supports the diphyletic 
      origin of H. murinum and H. secalinum. The in situ experiments yielded the 
      following conclusions: (1) differences between the sub-species marinum and 
      gussoneanum; (2) close relationships between the subspecies murinum and 
      leporinum; and (3) major differences in physical mapping between H. bulbosum and 
      the remaining taxa. The genomic and phylogenetic relationships between taxa, as 
      inferred from the results, are discussed.
FAU - de Bustos, A
AU  - de Bustos A
AD  - Department of Plant Breeding, CIT, INIA, La Canaleja, Madrid, Spain.
FAU - Cuadrado, A
AU  - Cuadrado A
FAU - Soler, C
AU  - Soler C
FAU - Jouve, N
AU  - Jouve N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 26S)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Classification
MH  - DNA Probes
MH  - DNA, Ribosomal/*genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF02261776 [doi]
PST - ppublish
SO  - Chromosome Res. 1996 Nov;4(7):491-9. doi: 10.1007/BF02261776.