PMID- 8939362
OWN - NLM
STAT- MEDLINE
DCOM- 19970227
LR  - 20191101
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 4
IP  - 7
DP  - 1996 Nov
TI  - A light microscopic atlas of meiosis in Arabidopsis thaliana.
PG  - 507-16
AB  - An atlas of meiosis in Arabidopsis thaliana, encompassing all stages from 
      preleptotene to telophase II and early microspore formation, is presented in 
      detail for the first time. The photomicrographs and descriptions are based on 
      staining with the DNA fluorochrome 4',6-diamidino-2-phenylindole (DAPI) combined 
      with a spreading procedure, or haematoxylin-iron alum (HIA) staining. Despite 
      previous reservations about the practicality of cytogenetic meiotic analysis in 
      Arabidopsis due to its small genome size, good-quality and clearly analysable 
      preparations of all meiotic stages were obtained. This atlas of normal, wild-type 
      meiosis is considered an essential prerequisite to informed analyses of meiotic 
      mutants. Furthermore, extended prophase I chromosomes, particularly at the 
      pachytene stage, offer considerable potential for producing a detailed 
      cytogenetic map (karyotype) of Arabidopsis chromosomes with the additional 
      prospect of high-resolution physical mapping based on fluorescence in situ 
      hybridization (FISH) of defined DNA probes to these extended chromosomes.
FAU - Ross, K J
AU  - Ross KJ
AD  - School of Biological Sciences, University of Birmingham, Edgbaston, UK.
FAU - Fransz, P
AU  - Fransz P
FAU - Jones, G H
AU  - Jones GH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Coloring Agents)
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - Anaphase/physiology
MH  - Arabidopsis/*metabolism
MH  - Coloring Agents/metabolism
MH  - DNA Probes
MH  - Fluorescent Dyes
MH  - Hematoxylin/metabolism
MH  - Indoles/metabolism
MH  - Meiosis/*physiology
MH  - Metaphase/physiology
MH  - Microscopy, Fluorescence
MH  - Prophase/physiology
MH  - Staining and Labeling
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF02261778 [doi]
PST - ppublish
SO  - Chromosome Res. 1996 Nov;4(7):507-16. doi: 10.1007/BF02261778.