PMID- 8940435
OWN - NLM
STAT- MEDLINE
DCOM- 19970328
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 34
IP  - 12
DP  - 1996 Dec
TI  - Characterization of Mycobacterium tuberculosis complex direct repeat sequence for 
      use in cycling probe reaction.
PG  - 2985-9
AB  - Cycling probe technology (CPT) is a unique and simple method for the detection of 
      specific target sequences. CPT utilizes a chimeric DNA-RNA-DNA probe providing an 
      RNase H-sensitive scissile linkage when bound to a complementary target sequence. 
      For this study a diagnostic assay based on CPT was developed for the detection of 
      the 36-bp direct repeat (DR) region in Mycobacterium tuberculosis. To determine 
      the feasibility of using the DR for detecting M. tuberculosis by CPT, a wide 
      variety of mycobacteria were tested by Southern blot hybridization with three DR 
      probes to verify their specificity. The entire DR region of Mycobacterium bovis 
      401 was sequenced, and the data were used to design a PCR assay that would allow 
      us to estimate the number of DRs present in a variety of strains. A CPT assay 
      which uses a probe complementary to the DR region was developed and evaluated 
      with synthetic targets and genomic DNA from mycobacteria. In summary, the 36-bp 
      DR provides an attractive target for detecting M. tuberculosis because the 
      sequence is present in high copy numbers in the genome, is specific for the M. 
      tuberculosis complex, and is found in strains that lack IS6110.
FAU - Beggs, M L
AU  - Beggs ML
AD  - Department of Pathology, University of Arkansas for Medical Sciences, Little 
      Rock, USA. MLBEGGS@life.uams.edu
FAU - Cave, M D
AU  - Cave MD
FAU - Marlowe, C
AU  - Marlowe C
FAU - Cloney, L
AU  - Cloney L
FAU - Duck, P
AU  - Duck P
FAU - Eisenach, K D
AU  - Eisenach KD
LA  - eng
SI  - GENBANK/U47864
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Probes)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Bacteriological Techniques/statistics & numerical data
MH  - Base Sequence
MH  - Cattle
MH  - DNA Probes/genetics
MH  - DNA Transposable Elements/genetics
MH  - DNA, Bacterial/genetics
MH  - Evaluation Studies as Topic
MH  - Genome, Bacterial
MH  - Humans
MH  - *Molecular Probe Techniques/statistics & numerical data
MH  - Molecular Sequence Data
MH  - Mycobacterium bovis/genetics
MH  - Mycobacterium tuberculosis/*genetics
MH  - Polymerase Chain Reaction
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sensitivity and Specificity
MH  - Species Specificity
PMC - PMC229446
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
PMCR- 1996/12/01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PHST- 1996/12/01 00:00 [pmc-release]
AID - 10.1128/jcm.34.12.2985-2989.1996 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1996 Dec;34(12):2985-9. doi: 10.1128/jcm.34.12.2985-2989.1996.