PMID- 8941376
OWN - NLM
STAT- MEDLINE
DCOM- 19961230
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 74
IP  - 3
DP  - 1996
TI  - High-resolution FISH of the entire integrated Epstein-Barr virus genome on 
      extended human DNA.
PG  - 211-7
AB  - Here we report a high-resolution fluorescence in situ hybridization (FISH) 
      analysis of the integrated Epstein-Barr virus (EBV) genome in chromosomes, 
      decondensed interphase nuclear chromatin, and linearly extended chromatin fibers. 
      We analyzed the EBV DNA integrated into the human genome in the 
      well-characterized Burkitt's lymphoma cell line Namalwa, which contains two 
      complete EBV genomes. The integration occurs via the terminal repeats of the 
      virus and was always detectable at chromosome band 1p35. Using the biotinylated 
      BamHIW fragment of the viral DNA, we observed distinct pairs of signals or small 
      nuclear RNA "tracks" within interphase nuclei. FISH to stretched DNA fibers has a 
      higher resolving power and; therefore, enables analysis of the structural 
      organization of DNA. Application of this methodology to linearly extended 
      chromatin of Namalwa cells using different EBV fragments allowed us to visualize 
      the ordered arrangement of the integrated virus. Based on the predicted span of 
      0.34 nm per base pair for relaxed DNA, length measurements of 30 images showed a 
      good correlation between the mean physical length of hybridized EBV DNA of 52.8 
      microns (158 kb) without the terminal repeats, and the EBV genomic length of 172 
      kb, including the terminal repeats. This DNA mapping procedure represents a 
      useful tool for studying the structural organization of integrated viral genomes, 
      and its application will have implications for the understanding of integration 
      processes.
FAU - Lestou, V S
AU  - Lestou VS
AD  - Children's Cancer Research Institute, St. Anna Kinderspital, Vienna, Austria.
FAU - Strehl, S
AU  - Strehl S
FAU - Lion, T
AU  - Lion T
FAU - Gadner, H
AU  - Gadner H
FAU - Ambros, P F
AU  - Ambros PF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Burkitt Lymphoma/virology
MH  - Chromosomes, Human, Pair 1/genetics/*virology
MH  - DNA, Viral/*analysis
MH  - *Genome, Viral
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Tumor Cells, Cultured
MH  - Virus Integration/*genetics
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134416 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;74(3):211-7. doi: 10.1159/000134416.