PMID- 8943048
OWN - NLM
STAT- MEDLINE
DCOM- 19970116
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 24
DP  - 1996 Nov 26
TI  - The mouse mammary tumor virus env gene is the source of a CD8+ T-cell-stimulating 
      peptide presented by a major histocompatibility complex class I molecule in a 
      murine thymoma.
PG  - 13991-6
AB  - CD8+ cytotoxic T cells recognize their targets by the presence of unique peptide 
      bound to a major histocompatibility complex (MHC) class I molecules on the cell 
      surface. The MHC molecules normally display thousands of distinct peptides, 
      making it difficult to identify individual antigenic peptides, their protein 
      precursors, and their relative importance in the T-cell response. Here we used 
      the EL-4 tumor-specific lacZ-inducible KZ30.6 T cell as a probe for detecting the 
      peptide/MHC ligand that was generated in cells transfected with an EL-4 cDNA 
      library. These expression screens allowed identification of a mouse mammary tumor 
      virus (MMTV) transcript as the source of the antigenic peptide presented by the 
      Kb MHC molecule. The antigenic activity was encoded within the MMTV env gene and 
      was defined by the octapeptide ANYDFICV (AFV8). Synthetic AFV8 stimulated KZ30.6 
      T cells at picomolar concentrations and coeluted with one of two active peptides 
      in HPLC-fractionated extracts of EL-4 cells. The AFV8/Kb complex was also 
      recognized by two other EL-4-specific T cells. The results illustrate a novel 
      strategy for identifying T-cell-stimulating antigens and suggest that the MMTV 
      env gene and its naturally processed AFV8 peptide product can serve as a model 
      for study of antigen processing and tumor immunotherapy.
FAU - Malarkannan, S
AU  - Malarkannan S
AD  - Department of Molecular and Cell Biology, University of California, Berkeley 
      94720, USA.
FAU - Serwold, T
AU  - Serwold T
FAU - Nguyen, V
AU  - Nguyen V
FAU - Sherman, L A
AU  - Sherman LA
FAU - Shastri, N
AU  - Shastri N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Gene Products, env)
RN  - 0 (H-2 Antigens)
RN  - 0 (Oligopeptides)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Gene Library
MH  - Gene Products, env/immunology
MH  - *Genes, env
MH  - H-2 Antigens/*immunology
MH  - Mammary Tumor Virus, Mouse/*genetics/*immunology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Oligopeptides/immunology
MH  - Recombinant Proteins/immunology
MH  - Thymoma/*immunology
MH  - Thymus Neoplasms/*immunology
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC19482
EDAT- 1996/11/26 00:00
MHDA- 1996/11/26 00:01
PMCR- 1997/05/26
CRDT- 1996/11/26 00:00
PHST- 1996/11/26 00:00 [pubmed]
PHST- 1996/11/26 00:01 [medline]
PHST- 1996/11/26 00:00 [entrez]
PHST- 1997/05/26 00:00 [pmc-release]
AID - 2689 [pii]
AID - 10.1073/pnas.93.24.13991 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13991-6. doi: 
      10.1073/pnas.93.24.13991.