PMID- 8943539
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 11
IP  - 10
DP  - 1996 Oct
TI  - Sex steroid regulation of leukocyte traffic in human decidua.
PG  - 2257-62
AB  - Endometrial leukocyte subpopulations vary over the reproductive cycle, but no 
      data exist on the mechanism regulating their recruitment into uterine tissue. 
      This study has evaluated the role of progesterone in the recruitment of selected 
      leukocyte populations in early pregnancy decidua. Decidua was collected from 
      women in early pregnancy at the time of vacuum aspiration of the uterus 6, 12, 24 
      and 36 h after taking 200 mg mifepristone (RU486). Standard immunohistochemical 
      techniques were employed to demonstrate the selected leukocyte populations in 
      decidual tissue and these were analysed using imaged analysis. Fresh decidua was 
      incubated in medium for 24 h and supernatants assayed for interleukin (IL-8) 
      (neutrophil chemotactic factor) and MCP-1 (monocyte chemoattractant protein-1) 
      content. Analysis of variance demonstrated a significant increase in tissue 
      monocyte number in decidua 12-36 h after mifepristone administration. No 
      significant changes in other leukocyte subpopulations were observed. Decidua IL-8 
      concentrations were significantly increased (P = 0.019) 6 h after mifepristone 
      and decidual MCP-1 concentration rose (non-significant) and fell significantly (P 
      = 0.029) between 6 and 12 h after mifepristone. Progesterone withdrawal may 
      initiate a local cascade of events involving inflammatory mediators which in turn 
      are responsible for the influx of monocytes. This influx may be essential in the 
      process of shedding of endometrium or decidua since monocytes and neutrophils are 
      important sources of proteases and collagenases. Furthermore, these cells are 
      potential local sources of immunomodulatory cytokines.
FAU - Critchley, H O
AU  - Critchley HO
AD  - Department of Obstetrics and Gynaecology, University of Edinburgh, UK.
FAU - Kelly, R W
AU  - Kelly RW
FAU - Lea, R G
AU  - Lea RG
FAU - Drudy, T A
AU  - Drudy TA
FAU - Jones, R L
AU  - Jones RL
FAU - Baird, D T
AU  - Baird DT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Hormone Antagonists)
RN  - 0 (Interleukin-8)
RN  - 320T6RNW1F (Mifepristone)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Decidua/*cytology/drug effects
MH  - Female
MH  - Hormone Antagonists/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-8/metabolism
MH  - Leukocyte Count
MH  - Leukocytes/*physiology
MH  - Macrophages/cytology
MH  - Mifepristone/pharmacology
MH  - Pregnancy
MH  - Progesterone/*physiology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a019086 [doi]
PST - ppublish
SO  - Hum Reprod. 1996 Oct;11(10):2257-62. doi: 10.1093/oxfordjournals.humrep.a019086.