PMID- 8943844
OWN - NLM
STAT- MEDLINE
DCOM- 19961231
LR  - 20240104
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 11
DP  - 1996 Dec 1
TI  - The t(9;14)(p13;q32) chromosomal translocation associated with lymphoplasmacytoid 
      lymphoma involves the PAX-5 gene.
PG  - 4110-7
AB  - The t(9;14)(p13;q32) translocation is associated with approximately 50% of 
      lymphoplasmacytoid lymphoma (LPL), a subtype of B-cell non-Hodgkin's lymphoma 
      (NHL). We cloned the chromosomal breakpoint of der (14) from an LPL case (1052) 
      and showed that it involved a junction between 9p13 and the switch micro region 
      of the Ig heavy chain locus (IgH) on 14q32. Using a YAC contig spanning 1.5 
      megabase (Mb), we determined that the 9p13 breakpoint in one case (1052) mapped 
      within a 270-kb restriction fragment containing two previously reported 9p 
      breakpoints associated with a alpha-heavy chain disease case (MAL) and KI-1 
      positive diffuse large cell lymphoma (DLCL) cell line (KIS-1). The same fragment 
      also contained the PAX-5 gene which encodes a B-cell specific transcription 
      factor involved in the control of B-cell proliferation and differentiation. The 
      breakpoints of KIS-1 and 1052 were mapped within the 5' noncoding region of 
      PAX-5, while the 9p13 breakpoint of MAL mapped 230 to 270 kb upstream to PAX-5. 
      In all three cases, the translocation caused the juxtaposition of the PAX-5 gene 
      to the IgH locus in the opposite direction of transcription. When compared with 
      six other DLCL cell lines lacking t(9;14)(p13;q32), the KIS-1 cell line showed an 
      11-fold overexpression of PAX-5 mRNA and a significantly reduced expression of 
      the p53 gene, which is normally regulated by PAX-5. Moreover, metaphase and 
      interphase fluorescence in situ hybridization (FISH) analysis using a YAC clone 
      spanning 1 Mb including the PAX-5 as a probe identified chromosomal 
      translocations in 5 of 7 cases carrying 9p13 translocations. These findings 
      suggest that the PAX-5 gene is the target of the t(9;14) in LPL whereby its 
      expression may be deregulated by juxtaposition to IgH regulatory elements, thus 
      contributing to lymphomagenesis.
FAU - Iida, S
AU  - Iida S
AD  - Department of Pathology, College of Physicians and Surgeons, Columbia University, 
      New York, NY 10032, USA.
FAU - Rao, P H
AU  - Rao PH
FAU - Nallasivam, P
AU  - Nallasivam P
FAU - Hibshoosh, H
AU  - Hibshoosh H
FAU - Butler, M
AU  - Butler M
FAU - Louie, D C
AU  - Louie DC
FAU - Dyomin, V
AU  - Dyomin V
FAU - Ohno, H
AU  - Ohno H
FAU - Chaganti, R S
AU  - Chaganti RS
FAU - Dalla-Favera, R
AU  - Dalla-Favera R
LA  - eng
SI  - GENBANK/U62539
GR  - CA-34775/CA/NCI NIH HHS/United States
GR  - CA-44029/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PAX5 Transcription Factor)
RN  - 0 (PAX5 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Transformation, Neoplastic/genetics
MH  - Chromosomes, Human, Pair 14/genetics/*ultrastructure
MH  - Chromosomes, Human, Pair 9/genetics/*ultrastructure
MH  - Cloning, Molecular
MH  - DNA, Neoplasm/genetics
MH  - DNA-Binding Proteins/*genetics/physiology
MH  - Exons/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/physiology
MH  - Nuclear Proteins/*genetics/physiology
MH  - PAX5 Transcription Factor
MH  - *Transcription Factors
MH  - *Translocation, Genetic
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0006-4971(20)61153-3 [pii]
PST - ppublish
SO  - Blood. 1996 Dec 1;88(11):4110-7.