PMID- 8946192
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20111117
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 17
IP  - 3
DP  - 1996 Nov
TI  - Demonstration of the translocation der(16)t(1;16)(q12;q11.2) in interphase nuclei 
      of Ewing tumors.
PG  - 141-50
AB  - The der(16)t(1;16) has been detected cytogenetically in a number of malignancies 
      including Ewing tumors (ETs). To enable fast and reliable analysis of der(16) 
      chromosomes, we established an interphase cytogenetic approach. By using two DNA 
      probes hybridizing to the heterochromatic portions on the long arms of 
      chromosomes 1 and 16, this technique allows the detection of this chromosomal 
      aberration in nonproliferating cells. Formation of the der(16) leads to partial 
      excess of 1q material and partial loss of the long arm of chromosome 16. 
      Double-target fluorescence in situ hybridization (FISH) experiments were 
      performed on cytospin slides of 13 ETs, near-triploid tumor cells and normal 
      cells to assess whether the FISH technique used permits the discrimination of 
      nuclei harboring this aberration from nuclei without a der(16) chromosome. In 
      five ETs, we found evidence for the presence of one or two der(16)t(1;16) 
      chromosomes both by FISH and by conventional cytogenetics. Tumor cells displayed 
      two signals for intact chromosomes 1, one or two additional fused signals for the 
      der(16) chromosomes, and one signal for the intact chromosome 16. In one case 
      without fused signals, the presence of a der(16) was demonstrated by hybridizing 
      a painting probe for chromosome 16 simultaneously with the paracentromeric probe 
      for chromosome 1. Our results suggest that double-target FISH on interphase 
      nuclei offers an ideal tool for analyzing tumors prospectively and 
      retrospectively to assess the biological role and the possible prognostic impact 
      of the der(16) in ETs and in other solid tumors.
FAU - Hattinger, C M
AU  - Hattinger CM
AD  - CCRI, St. Anna Kinderspital, Vienna, Austria.
FAU - Rumpler, S
AU  - Rumpler S
FAU - Ambros, I M
AU  - Ambros IM
FAU - Strehl, S
AU  - Strehl S
FAU - Lion, T
AU  - Lion T
FAU - Zoubek, A
AU  - Zoubek A
FAU - Gadner, H
AU  - Gadner H
FAU - Ambros, P F
AU  - Ambros PF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bone Neoplasms/*genetics
MH  - Child
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 16
MH  - DNA Probes/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Sarcoma, Ewing/*genetics
MH  - *Translocation, Genetic
EDAT- 1996/11/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199611)17:3<141::AID-GCC1>3.0.CO;2-4 [pii]
AID - 10.1002/(SICI)1098-2264(199611)17:3<141::AID-GCC1>3.0.CO;2-4 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Nov;17(3):141-50. doi: 
      10.1002/(SICI)1098-2264(199611)17:3<141::AID-GCC1>3.0.CO;2-4.