PMID- 8946195
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 17
IP  - 3
DP  - 1996 Nov
TI  - Identification of a yeast artificial chromosome spanning the 8q12 translocation 
      breakpoint in pleomorphic adenomas with t(3;8)(p21;q12).
PG  - 166-71
AB  - A subgroup of pleomorphic adenomas of the salivary glands is characterized by 
      translocations involving chromosome 8, with consistent breakpoints at 8q12. As 
      part of a positional cloning effort to isolate the gene(s) affected by these 
      translocations we now report the mapping of the 8q12 breakpoint in two primary 
      pleomorphic adenomas with the recurrent t(3;8)(p21;q12). Yeast artificial 
      chromosome (YAC) clones corresponding to eight different loci in 8q11-12 were 
      isolated and mapped by fluorescence in situ hybridization (FISH). The t(3;8) 
      breakpoint was mapped within a 1 Mb region flanked by MOS proximally and by the 
      genetic marker D8S166 distally. One YAC within this region was shown to span the 
      t(3;8) breakpoint in two tumors. This YAC will provide an excellent tool for 
      isolating the gene(s) at the breakpoint(s) in adenomas with t(3;8).
FAU - Roijer, E
AU  - Roijer E
AD  - Department of Pathology, Goteborg University, Sweden.
FAU - Kas, K
AU  - Kas K
FAU - Klawitz, I
AU  - Klawitz I
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Van de Ven, W
AU  - Van de Ven W
FAU - Stenman, G
AU  - Stenman G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adenoma, Pleomorphic/*genetics
MH  - Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 3
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Salivary Gland Neoplasms/*genetics
MH  - *Translocation, Genetic
EDAT- 1996/11/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199611)17:3<166::AID-GCC4>3.0.CO;2-2 [pii]
AID - 10.1002/(SICI)1098-2264(199611)17:3<166::AID-GCC4>3.0.CO;2-2 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Nov;17(3):166-71. doi: 
      10.1002/(SICI)1098-2264(199611)17:3<166::AID-GCC4>3.0.CO;2-2.