PMID- 8946202
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20060406
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 17
IP  - 4
DP  - 1996 Dec
TI  - Molecular cytogenetic characterization and physical mapping of 12q13-15 
      amplification in human cancers.
PG  - 205-14
AB  - Amplification of sequences derived from 12q13-15 is frequent in human sarcomas 
      and brain tumors. Detailed mapping studies of the amplified region are necessary 
      for definition of the impact of these amplification events on the tumor cell 
      phenotype. By using the genes in this region and genomic fragments isolated by 
      chromosome microdissection, we have established a series of ordered probes from 
      12q13-15 for fluorescence in situ hybridization (FISH) and Southern blot 
      analysis. These probes have been used for physical mapping of two portions of the 
      interval from GLI to D12S8. The centromeric region extends 1.8 Mb from GLI to 
      microclone M79 and contains at least five genes, including the cyclin-dependent 
      kinase gene CDK4. The more telomeric region includes the p53 regulator MDM2 and 
      covers 1.1 Mb. We used the same group of probes to determine the pattern of 
      amplification in three cell lines and three tumor specimens carrying amplified 
      sequences from 12q13-15. In addition, we used a yeast artificial chromosome (YAC) 
      contig of several megabases covering the entire region from SAS to D12S8 for FISH 
      to determine the pattern of amplification in the neuroblastoma cell line NGP-127. 
      The results suggest that the MDM2 and CDK4 regions may be either coamplified or 
      amplified independently, and they illustrate how the map positions of genes and 
      their functions may interact to determine the pattern of DNA amplification in 
      human malignancies.
FAU - Elkahloun, A G
AU  - Elkahloun AG
AD  - Laboratory of Cancer Genetics, National Center for Human Genome Research, 
      National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
FAU - Bittner, M
AU  - Bittner M
FAU - Hoskins, K
AU  - Hoskins K
FAU - Gemmill, R
AU  - Gemmill R
FAU - Meltzer, P S
AU  - Meltzer PS
LA  - eng
SI  - GENBANK/J00219
SI  - GENBANK/M23657
SI  - GENBANK/M68250
SI  - GENBANK/X12533
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 12
MH  - *Gene Amplification
MH  - Histiocytoma, Benign Fibrous/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Neuroblastoma/*genetics
MH  - Rhabdomyosarcoma/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1996/12/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199612)17:4<205::AID-GCC2>3.0.CO;2-7 [pii]
AID - 10.1002/(SICI)1098-2264(199612)17:4<205::AID-GCC2>3.0.CO;2-7 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Dec;17(4):205-14. doi: 
      10.1002/(SICI)1098-2264(199612)17:4<205::AID-GCC2>3.0.CO;2-7.