PMID- 8946279
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20190920
IS  - 0271-9142 (Print)
IS  - 0271-9142 (Linking)
VI  - 16
IP  - 6
DP  - 1996 Nov
TI  - Antiplatelet glycoprotein autoantibodies in patients with autoimmune diseases 
      with and without thrombocytopenia.
PG  - 340-7
AB  - The presence and specificity of antiplatelet autoantibodies in 32 patients with 
      primary and 18 patients with secondary autoimmune thrombocytopenic purpura 
      (AITP), as well as 11 non-thrombocytopenic patients with systemic autoimmune 
      diseases, were studied. By means of the direct and indirect monoclonal antibody 
      immobilization of platelet antigen (MAIPA) assay, antiplatelet autoantibodies 
      were detected using monoclonal antibodies specific for platelet glycoproteins 
      (GPs) Ib, IIb/IIIa, Ia/IIa, and IV. Serum antiplatelet autoantibodies were found 
      in 18 of 32 primary AITP patients (56%), 6 of 18 secondary AITP patients (33%), 
      and 5 of 11 nonthrombocytopenic patients (45%). Platelet-associated 
      autoantibodies were detected in five of eight patients with primary (62%) and in 
      four of eight patients with secondary AITP (50%) and in two of four patients 
      without thrombocytopenia (50%). Multiple antibody reactivity, mainly against GPs 
      IIb/IIIa and Ib and, in a few patients, against Ia/IIa, was found. Using MAIPA, 
      platelet xylene eluates from 20 patients were also studied. Antiplatelet elutable 
      autoantibodies were related to thrombocytopenia; autoantibodies against membrane 
      GPs Ib and IIb/IIIa were demonstrable in 84 and 63% of eluates from patients with 
      primary and secondary AITP, respectively, but not in eluates from 
      nonthrombocytopenic patients. The presence of antiplatelet antibodies thus 
      appears to be a common feature of many autoimmune diseases apart from the 
      thrombocytopenia, but the (primary or secondary) etiology of the immune 
      thrombocytopenia cannot be differentiated on the grounds of their specificity.
FAU - Cordiano, I
AU  - Cordiano I
AD  - Institute of Medical Semeiotics, University of Padua Medical School, Italy.
FAU - Salvan, F
AU  - Salvan F
FAU - Randi, M L
AU  - Randi ML
FAU - Ruffatti, M A
AU  - Ruffatti MA
FAU - Steffan, A
AU  - Steffan A
FAU - Girolami, A
AU  - Girolami A
FAU - Fabris, F
AU  - Fabris F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Autoantibodies/*analysis
MH  - Autoantigens/immunology
MH  - Autoimmune Diseases/complications/*immunology
MH  - Blood Platelets/*immunology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/*immunology
MH  - Purpura, Thrombocytopenic, Idiopathic/complications/*immunology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF01541670 [doi]
PST - ppublish
SO  - J Clin Immunol. 1996 Nov;16(6):340-7. doi: 10.1007/BF01541670.