PMID- 8946481
OWN - NLM
STAT- MEDLINE
DCOM- 19970324
LR  - 20220316
IS  - 0031-9384 (Print)
IS  - 0031-9384 (Linking)
VI  - 60
IP  - 6
DP  - 1996 Dec
TI  - Electrode positioning for reliable telemetry ECG recordings during social stress 
      in unrestrained rats.
PG  - 1397-401
AB  - We describe a surgical procedure for optimizing the location of telemetry ECG 
      leads in rats. The new location was aimed at obtaining an accurate representation 
      of ECG features throughout the cardiac cycle by limiting the voltage instability 
      usually observed during intense somatomotor activity and improving the 
      signal-to-noise ratio. The two electrodes (wire loops) were fixed on the dorsal 
      surface of the xiphoid process and in the anterior mediastinum close to the right 
      atrium. The implantation procedure was fast, little invasive, and allowed animals 
      to completely recover from intervention. The performance of the "improved" 
      location (IL, n = 10) with respect to two subcutaneous (SC) positionings 
      ("conventional positioning" CSP, n = 5; "updated location," USL, n = 5) was 
      evaluated by comparing ECGs obtained in baseline, stress and recovery conditions 
      and during different behavioral activities (immobility and grooming). The 
      resident-intruder test (emotional/physical challenge) was chosen as experimental 
      stress paradigm. The noise level of ECGs obtained from IL rats was lower than in 
      CSP and USL animals, in all recording conditions. Percentages of correctly 
      recognized beats (CRBs) over the total number of beats (TBs) were significantly 
      higher in IL rats than in CSP and USL animals, both in baseline conditions (99% 
      vs. 11% and 40%) and situations involving high somatomotor activity (stress: 97%, 
      5% and 16% recovery; 97%, 7% and 15%) (p < 0.01). The performance of IL as 
      compared to CSP and USL was also better when percentages during grooming and 
      immobility were considered (grooming: 93% vs. 4% and 23%: immobility: 97%, 6%, 
      and 33%; p < 0.01).
FAU - Sgoifo, A
AU  - Sgoifo A
AD  - Dipartimento di Biologia Evolutiva e Funzionale, Universita di Parma, Italy.
FAU - Stilli, D
AU  - Stilli D
FAU - Medici, D
AU  - Medici D
FAU - Gallo, P
AU  - Gallo P
FAU - Aimi, B
AU  - Aimi B
FAU - Musso, E
AU  - Musso E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Physiol Behav
JT  - Physiology & behavior
JID - 0151504
SB  - IM
MH  - Animals
MH  - *Electrodes
MH  - Male
MH  - *Ovum
MH  - Rats
MH  - Rats, Wistar
MH  - Research Design
MH  - Stress, Physiological/*physiopathology
MH  - *Telemetry
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0031-9384(96)00228-4 [pii]
AID - 10.1016/s0031-9384(96)00228-4 [doi]
PST - ppublish
SO  - Physiol Behav. 1996 Dec;60(6):1397-401. doi: 10.1016/s0031-9384(96)00228-4.